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題名 | Genetic Detection of Diarrheagenic Escherichia Coli Isolated from Children with Sporadic Diarrhea=以基因檢測法測定腹瀉兒童之腸道致病性大腸桿菌 |
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作者 | 鄧麗珍; 薛博仁; 廖淑貞; 何憲武; 蔡瑞章; Teng, Lee-jene; Hsueh, Po-ren; Liaw, Shwu-jen; Ho, Shen-wu; Tsai, Jui-chang; |
期刊 | 微免與感染雜誌 |
出版日期 | 20041200 |
卷期 | 37:6 2004.12[民93.12] |
頁次 | 頁327-334 |
分類號 | 414.83 |
語文 | eng |
關鍵詞 | 基因檢測法; 腹瀉; 兒童; 大腸桿菌; Diarrhea; Escherichia coli; PCR; Preschool child; |
英文摘要 | Escherichia coli strains are among the major bacterial causes of diarrheal illness. At least 5 categories of diarrheagenic E. coli (DEC) are recognized, namely enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), and enterohemorrhagic E. coli (EHEC). Due to the need for costly and labor-intensive diagnostic procedures, identification of DEC is difficult at standard laboratories. Therefore, the epidemiology of DEC infections remains obscure in Taiwan. Recently, polymerase chain reaction (PCR) or dot blot has been used for genetic detection of DEC. In this study, we analyzed 150 E. coli isolates from diarrheal stools of children under 5 years old. The PCR tests detected 5 ETEC (3.3%), 6 EPEC (4%), 4 EIEC (2.7%), and 13 EAEC (8.7%) isolates. No EHEC was detected. Dot blot and sequence analysis were used to confirm the results of PCR. The cellular fatty acid (CFA) profiles from E. coli isolates were also analyzed. Comparison of CFA composition revealed minor variation in the percentage of each fatty acid detected among DEC isolates of ETEC, EPEC, EIEC and EAEC, but did not provide enough evidence for differentiating between categories of DEC by CFA profiles alone. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。