頁籤選單縮合
題 名 | IgA Immune Complex Blunts the Contraction of Cultured Mesangial Cells through the Inhibition of Protein Kinase C and Intracellular Calcium |
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作 者 | Jin,Jong-shiaw; Yao,Chen-wen; Ka,Shuk-man; Chin,Ting-yu; Chueh,Sheau-huei; Lee,Herng-sheng; Sheu,Lai-fa; Lin,Yeh-feng; Lee,Wei-hwa; Chen,Ann; | 書刊名 | 中國生理學雜誌 |
卷 期 | 47:2 2004.06[民93.06] |
頁 次 | 頁79-87 |
分類號 | 415.1461 |
關鍵詞 | Mesangial cells; Contractility; Protein kinase C; Intracellular calcium; |
語 文 | 英文(English) |
英文摘要 | The effects of IgA immune complex (IgA-IC) on the contractile function of cultured mesangial cells were measured by the changes in planar surface area in response to treatment with agonists. Incubation of mesangial cells with IgA-IC for 24 hours significantly decreased the contractile responses to angiotensin Ⅱ(10⁻⁶ M) and phorbol 12-myristate 13-acetate (PMA, 10⁻⁶ M). Pretreatment of mesangial cells with the protein kinase C (PKC) inhibitor, chelerythrine (10⁻⁶ M), eliminated the difference in contractile responses to angiotensin II or PMA between the control and IgA-IC groups indicating IgA-IC may inhibit the activity of PKC. The contractile responses to ionomycin were not significantly different between IgA-IC treated and control mesangial cells, suggesting that the contractile machinery is not impaired by IgA-IC. Intracellular calcium, [Ca²⁺]i measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells. In contrast, treatment with thapsigargin did not result in significant differences in [Ca²⁺]i between IgA-IC and control mesangial cells, suggesting that a negligible role of endoplasmic reticulum in the effects of IgA-IC. Using PKC specific antibodies, IgA-IC significantly increased the particulate fraction of PKC-l of mesangial cells to 141±13% of control, without significantly changing the protein content of PKC-α, -δ and -λ in the cytosolic and particulate fractions. In summary, IgA-IC inhibits the contractile responses of cultured mesangial cells to agonists by inhibiting the activation of PKC and [Ca²⁺]i. |
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