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題 名 | 新城雞瘟活毒疫苗投予後病毒在雞體內分布及其對免疫反應之影響=The Distribution and Vicissitude of Newcastle Disease Live Vaccine Virus in Chickens and Its Influence on Immune Response |
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作 者 | 謝快樂; 曾啟文; 沈瑞鴻; 李龍湖; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 23:5 1997.10[民86.10] |
頁 次 | 頁450-462 |
分類號 | 437.714 |
關鍵詞 | 新城雞瘟; 病毒分布; 反轉錄─聚合酶連鎖反應; 免疫反應; Newcastle disease; Virus distribution; Polymerase chain reaction; Immune response; |
語 文 | 中文(Chinese) |
中文摘要 | 為了解新城雞瘟活毒疫苗免疫後,病毒在雞隻體內的分布及消長情形,採用反轉 錄-聚合�t連鎖反應 (RT-PCR) 來進行病毒核酸的偵測本實驗使用針對己發表的新城雞瘟病 毒 B1 株核酸序列所設計的特異性引子,在確定其特異性後,結合反轉錄作用與聚合�t連鎖 反應, 將之化於一個反應管中進行,來偵測新城雞瘟病毒 (NDV) 在免疫後於雞隻體內的分 布及消長。結果顯示,SPF 雞隻以活毒疫苗免疫後,可偵測到病毒核酸的時間較帶有移行抗 體的雞隻為長;就所能偵測到的分布區域而言,則明顯與疫苗投予的方式有關。在點眼、口 服、噴霧三種疫苗投予方式中,以點眼方式免疫的雞隻,病毒在體內分布的部位皆為三種方 式中最為平均而且持續時間最久的,其中又以免疫組織器官所能檢測到病毒核酸的時間最長 。以口服方式投予,病毒分布局限於上消化道,能檢出病毒核酸的時間則為三種方式之末。 至於噴霧免疫的方式,病毒同樣呈現局部分布的情形,以上呼吸道為主,檢出病毒核酸的時 間則有些許的差異。以上述實驗結果為基礎所進行的雞隻免疫抗體力價測定實驗,採行點眼、 口服、噴霧、皮下注射等方式投予新城雞瘟活毒疫苗。在基礎免疫後,各組抗體力價的變化 情形差異很小,但經過油劑疫苗補強免疫之後,以點眼方式進行基礎免疫的雞隻抗體上升的 速度最快,程度最高且均勻度最好;噴霧免疫次之,皮下注射方式則其高抗體力價持續時間 最長,而以口服免疫效果最差。 |
英文摘要 | A single tube reverse transcription-polymerase chain reaction (RT-PCR) was developed to evaluate the distribution and vicissitude of Newcastle disease virus (NDV)in chicken vaccinated by live vaccines. A primer set framed a region within the gene coding for fusion protein of NDV strain B1 was selected for RT-PCR. The result showed that, after vaccination, the presence of NDV RNA in specific pathogenic free chickens was longer than that in maternal antibody-containing chickens. In addition, the location of viral RNA was dependent on the routes of vaccination. The eye-drop administration not only generated the widest range fo viral RNA distribution but also gave the longest period of the presence of viral RNA. Moreover, after the eye-drop administration, NDV RNA could be detected for the longest period in the lymphoid tissues than in any other tissues. On the other hand, viral RNA distribution was only limited to the upper respiratory tract, and the period of the presence of viral RNA was short following oral administration. Similar result was obtained with aerosal vaacination, but the period of the presence of viral RNA was different. In primary immunization, the titers of antibody to NDV generated by different administration routes (eye-drop, oral, aerosol and subcutaneous) exhibited no significant difference. However, after the boost immunization by oil-emulsified vaccine, the highest increasing rate of antibody titer was found in the birds vaccinated by eye-drop administration followed by the aerosol administration in primary immunization. The longest period of high antibody titer was found in birds immunized subcutaneously. The poorest result of immune response was obtained from the birds vacinated orally. |
本系統中英文摘要資訊取自各篇刊載內容。