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題名 | Effect of Diethylstilbestrol on Ca[fec7] Handling and Cell Viability in Human Breast Cancer Cells |
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作者姓名(外文) | Chang,Hong-tai; Hsu,Shu-shong; Chen,Jin-shyr; Lin,Ko-long; Wang,Jue-long; Cheng,He-hsiung; Lu,Yih-chau; Jiann,Bang-ping; Liu,Chun-peng; Huang,Jong-khing; Yeh,Jeng Hsien; Chiang,An-jen; Chen,Wei-chuan; Jan,Chung-ren; | 書刊名 | 中國生理學雜誌 |
卷期 | 46:4 2003.12[民92.12] |
頁次 | 頁187-192 |
分類號 | 416.226 |
關鍵詞 | Breast cancer cells; Ca[fec7]; Ca[fec7] stores; Diethylstilbestrol; Fura-2; |
語文 | 英文(English) |
英文摘要 | In human breast cancer cells, the effect of the widely prescribed estrogen diethylstilbestrol (DES) on intracellular Ca2+ concentrations ([Ca²⁺]i) and cell viability was explored by using fura-2 and trypan blue exclusion, respectively. DES caused a rise in [Ca²⁺]i in a concentration-dependent manner (EC50=15 μM). DES-induced [Ca²⁺]i rise was reduced by 80 % by removal of extracellular Ca²⁺. DES-induced Mn²⁺-associated quench of intracellular fura-2 fluorescence also suggests that DES induced extracellular Ca²⁺ influx. In Ca²⁺-free medium, thapsigargin, an inhibitor of the endoplasmic reticulum Ca²⁺-ATPase, caused a monophasic [Ca²⁺]i rise, after which the increasing effect of DES on [Ca²⁺]i was greatly inhibited. Conversely, pretreatment with DES to deplete intracellular Ca²⁺ stores totally prevented thapsigargin from releasing more Ca²⁺, whereas ionomycin added afterward still released some Ca²⁺. These findings suggest that in human breast cancer cells, DES increases [Ca²⁺]i by stimulating extracellular Ca²⁺ influx and also by causing intracellular Ca²⁺ release from the endoplasmic reticulum. Acute trypan blue exclusion studies suggest that 10-20 μM DES killed cells in a time-dependent manner. |
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