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題 名 | Identification of a Uteroglobin Homologous Transcript (UGRP1) Expressed in Rat Bronchiolar Epithelial Cells=鑑定大鼠支氣管上皮細胞之子宮球蛋白同源基因 |
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作 者 | 林恆毅; 王進賢; 盧志峰; | 書刊名 | 輔仁醫學期刊 |
卷 期 | 1:1 2003.12[民92.12] |
頁 次 | 頁21-33 |
分類號 | 415.114 |
關鍵詞 | 支氣管上皮細胞; 差異性基因表現; 同源基因; 抑制消減雜交法; 子宮球蛋白; Bronchiolar epithelial cells; Differential gene expression; Homolog; Suppression subtractive hybridization; Uteroglobin; |
語 文 | 英文(English) |
英文摘要 | Background and Purpose: Primary cultures of lung epithelium provide ideal models to study the pathophysiology underlying airway and pulmonary diseases. However, many bronchiolar and alveolar epithelial cells gradually lose their phenotypic properties and fail to maintain differentiated morphology and function under conventional culture conditions. Differential gene expression has been shown to be involved in many biological processes including cell differentiation. To understand the molecular mechanism involved in the loss of differentiation, genes whose expression was modulated during in vitro culture of isolated rat lung epithelial cells were investigated. Methods: Suppression subtractive hybridization (SSH) takes advantage of subtractive hybridization and suppression PCR to detect differences between cDNA populations. SSH was used to selectively amplify differentially expressed transcripts which were enriched in freshly isolated compared to 4-day-cultured rat lung epithelial cells. The differential expression statuses of SSH-identified transcripts were further validated by Northern blot analysis. The tissue distribution of differentially expressed transcripts was also investigated by in situ hybridization and multiple tissue Northern blot analysis. Results: A novel 0.6-kb transcript encoding a homolog of rat uteroglobin (UGRP1) was identified by SSH. The expression of UGRP1 mRNA was enriched ni freshly isolated rat lung epithelial cells and diminished after 4 days of culture under conventional conditions based on Northern blot analysis. By in situ hybridization, transcripts of UGRP1 were detected only in bronchiolar epithelium and not in alveloar epithelium. UGRP1 mRNA was predominantly distributed in the lung as compared to other tissues according to multiple-tissue Northern blot analysis. Conclusions: Uteroglobin is a hormonally regulated secretory protein produced by the mucosal epithelia of various organs including the lung, uterus, prostate, and breast. Using SSH, we identified a novel uteroglobin homolog, UGRP1, whose expression was mainly detected in rat bronchiolar epithelial cells and was modulated during in vitro culture. Further characterization of UGRP1 is needed to clarify its role in the regulation of bronchiolar epithelial cell function. |
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