頁籤選單縮合
題 名 | Effect of Gossypol on Intracellular Ca[fec7] Regulation in Human Hepatoma Cells |
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作 者 | Cheng,Jin-shiung; Lo,Yuk-keung; Yeh,Jeng-hsien; Cheng,He-hsiung; Liu,Chun-peng; Chen,Wei-chuan; Jan,Chung-ren; | 書刊名 | 中國生理學雜誌 |
卷 期 | 46:3 2003.09[民92.09] |
頁 次 | 頁117-122 |
分類號 | 418.27 |
關鍵詞 | Ca[fec7] signaling; Fura-2; Gossypol; HA22/VGH cells; Hepatocytes; |
語 文 | 英文(English) |
英文摘要 | Gossypol is a natural toxicant present in cottonseeds, and is hepatotoxic to animals and human. The effect of gossypol on cytosolic free Ca²⁺ levels ([Ca²⁺]i) in HA22/VGH human hepatocytes was explored using fura-2 as a fluorescent Ca²⁺ indicator. Gossypol increased [Ca²⁺]i in a concentrationdependent manner with an EC50 value of 2 μM. The Ca²⁺ signal was reduced by removing extracellular Ca²⁺ or by 10 μM La³⁺, but was not affected by nifedipine, verapamil or diltiazem. Pretreatment with 1 μM thapsigargin (an endoplasmic reticulum Ca²⁺ pump inhibitor) to deplete the endoplasmic reticulum Ca²⁺ partly reduced 10 μM gossypol-induced Ca²⁺ release; and conversely pretreatment with gossypol abolished thapsigargin-induced Ca2+ release. The Ca²⁺ release induced by 10 μM gossypol was not changed by inhibiting phospholipase C with 2 μM U73122 or by depleting ryanodine-sensitive Ca²⁺ stores with 50 μM ryanodine. Together, the results suggest that in human hepatocytes, gossypol induced a [Ca²⁺]i increase by causing store Ca²⁺ release from the endoplasmic reticulum in a phospholipase Cindependent manner, and by inducing Ca²⁺ influx. |
本系統中英文摘要資訊取自各篇刊載內容。