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題 名 | 改良式免疫組織化學染色技術應用於福馬林液固定與石蠟包埋豬組織之研究= Modified Immunohistochemical Staining Employed in Formalin-Fixed, Paraffin-Embedded Porcine Tissue Sections |
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作 者 | 劉振軒; 王綉真; 蘇豐振; 傅千惠; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 23:6 1997.12[民86.12] |
頁 次 | 頁523-530 |
分類號 | 437.24 |
關鍵詞 | 豬; 免疫組織化學染色; 微波加熱處理; Porcine; Immunohistochemistry; Microwave heating; M4icroProbe[fef0]; |
語 文 | 中文(Chinese) |
中文摘要 | 使用福馬林液固定與石蠟包埋之切片標本,以Avidin Biotinylated enzyme Complex(ABC)行免疫組織化學染色,常有費時過長且使組織抗原性不活化,因而降低染色 品質之缺點,針對此缺失,乃進行改良式染色法研究以資改進。本研究係耐將豬大腦、小腦 、肺、脾、肝、皮膚、心肌、淋巴結及腎等組織, 經 10% 福馬林液固定與石蠟包埋切片後 ,配合 MicroProbe �蟧鷎馱庢L波烤箱加熱處理,進行 anti-human cytokeratin AE1/AE3, desmin, anti-swine vimentin, S-100 protein, anti-human glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE)FIL-4(neurofilament 70 kd&200 kd), factor VIII related antigen 等八種抗體 ABC 免疫組織化學染色, 並與傳統式不用 MicroPorbe �蟧鷎劂P微波加熱處理的免疫組織化學染色作比較。 結果顯示改良式免疫組織 化學染色花費時間約需 2-2.5 小時,遠較傳統式花費時間需過夜又 5 小時,不僅更具時效 性, 而且節省試劑與抗體之用量。 在染色效果之比較, 除了 S-100 protein 及 factor VIII related antigen 改良式與傳統式並無顯著差異外, 其餘 anti-human cytokeratin AE1/AE3, desmin, anti-swine vimentin, GFAP, NSE 及 FIL-4 (neurofilament 70 kd & 200 kd),改良式染色效果均較傳統式為佳。 由上述實驗比較改良式與傳統式免疫組織化學 染色結果,初步證實上述八種市售抗體均可適用上述兩種染色方法而應用於豬組織,使用改 良式由於節省時間,染色效果穩定,所以是一種可行、方便並能爭取時效的免疫組織化學染 色方法。 [* 劉振軒、王�銡u、蘇豐振、傅千惠。改良式免疫組織化學染色技術應用於福馬 林液固定與石蠟包埋豬組織之研究。 中華獸醫誌 23(6) : 523-530, 1997。 * 聯絡人 TEL:037-672352 轉 507, FAX:037-680478] |
英文摘要 | Avidin Biotinylated enzyme Complex (ABC) immunohistochemistry employed in the formalin-fixed, paraffin-embedded porcine tissues is commonly seen. The disadvantages of formalin fixative and conventional staining method are antigen inactivation, time-consumption and reagent waste. For the purpose of improvement of staining effect and efficacy, we developed a modified staining method to compare with conventional staining method. In the modified method, 10% formalin-fixed porcine tissues including cerebrum, cerebellum, lung, liver, shin, myocardium, lymph modes, and kidney were used, and ABC immunohistochemical staining of anti-human cytokeratin AE1/AE3, desmin, anti-swine vimentin, S-100 protein, anti-human glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE), FIL-4 (neurofilament 70 kd & 200 kd), and factor VIII related antigen were performed by using microwave heating treatment and MicroProbe ��. The conventional method used neither microwave heating treatment nor MicroProbe ��. The results showed that all above 8 market-available antibodies can be employed in porcine tissues by either modified or conventional method. However, the modified method took 2-2.5 hours compared with overnight of the conventional method. With respect to the staining effect, the modified method of anti-human cytokeratin AE1/AE3, desmin, antiswine vimentin, GFAP, NSE, and FIL-4 (neurofilament 70 kd & 200 kd) staining showed a stronger and brighter effect than those stained with the conventional method. In conclusion, the modified immunohistochemical method is a practical, convenient, and fast technique to obtain reliable results.[*Liu CH, Wang HC, Su FC and Fu CH. A modified immunohistochemical staining employed in formalin-fixed, paraffin-embedded porcine tissue sections. J Chin Soc Vet Sci 23(6):523-530,1997. *Corresponding author TEL:037-672352 ext 251, FAX:037-680478] |
本系統中英文摘要資訊取自各篇刊載內容。