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題 名 | 產蛋下降症病毒臺灣分離株診斷用核酸探針的建立=The Establishment on Diagnostic Nucleic Acid Probe for Egg Drop Syndrome Virus in Taiwan Isolates |
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作 者 | 林德田; 賴秀穗; 許天來; 蘇杰夫; 林士鈺; | 書刊名 | 臺灣省家畜衛生試驗所研究報告 |
卷 期 | 34 1998.12[民87.12] |
頁 次 | 頁21-29 |
分類號 | 437.246 |
關鍵詞 | 產蛋下降症; 核酸探針; Egg drop syndrome; Nucleic acid probe; |
語 文 | 中文(Chinese) |
中文摘要 | 為建立核酸探針檢測產蛋下降症病毒(Egg drop syndrome virus, EDSV),利用 pBlueScript Ⅱ KS (+/-) 載體進行 EDSV DNA 選值,獲得 8 個片段的選殖株, 其中末端 基因片段並未獲得。 選用 1.5 kilobase pairs 大小的選殖 DNA 片段做為核酸探針,並經 非放射性 Digoxigenin (DIG) 標識, 標識的核酸探針進行南方轉漬可以特異性的測出交株 EDS-76 病毒 (CO、NO.4、NO.5、 NO.7、NO.8 及 NO.18), 但無法檢測到家禽腺病毒 NO.9 株。利用南方轉漬及核酸雜合試驗可檢測到 100 pg 的 EDS-76 病毒 DNA 及檢測出 EDS 病 毒感染 1 至 25 小時鴨胚胎纖維母細胞之病毒 DNA,因此本核酸探針可應用於檢測 EDS 病 毒 DNA。 |
英文摘要 | In order to establish the diagnostic nucleic acid probe for detecting to egg drop syndrome virus (EDSV) DNA, the genome of EDSV CO strain isolated from chicken oviduct was cloned in pBlueScript Ⅱ KS (+/-)vector. Eight DNA fragments in EDSV were cloned, but terminal genomic DNA were not. A specific digoxigenin (DIG)--labeled probe was prepared from a 1.5 kilobase-pair-cloned fragment. Six EDSV Taiwan isolates were detected by the nucleic acid probe method, but one avian adenovirus (NO.9 strain) was not. The 100 pg volume of EDSV DNA and infected duck embryonated fibroblast (DEF) cells were surveyed by southern blotting and DNA hybridization. Thw established DNA probe technique of this study can be used as a detective tool for infection of EDSV. |
本系統中英文摘要資訊取自各篇刊載內容。