頁籤選單縮合
題 名 | Complex Formation between the Lumenal Domain of Adenovirus E3-19K Protein and the Extracellular Domain of Class I MHC Molecule in Vitro |
---|---|
作 者 | 廖平喜; 陳德祥; 廖珮妤; | 書刊名 | Journal of the Chinese Chemical Society |
卷 期 | 49:4 2002.08[民91.08] |
頁 次 | 頁607-610 |
分類號 | 346.77 |
關鍵詞 | Complex formation; Adenovirus E3-19k protein; Major histocompatibility complex; MHC; HLA-A*0201; |
語 文 | 英文(English) |
英文摘要 | The Ad2 E3-19k protein inhibits the transport of newly synthesized class I MHC molecules to the cell surface, thereby interfering with antigen presentation. The details of the interaction between E3-19k protein and class I MHC molecules have not been well-defined. In this present study, we describe the use of gel filtration HPLC for confirming the binding interaction of two domain proteins, E3-19k and MHC class I antigen, and subsequently the characterization of protein complex by SDS-PAGE. Our results demonstrate the complex formation between Ad2 lumenal E3-19k (108 amino acids, wt 108) and HLA-A*0201 molecule in vitro. Titration experiments will be employed in the future to determine stoichiometry and verify the specific interactions. interactions. |
本系統中英文摘要資訊取自各篇刊載內容。