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題名 | Purification of Candida Rugosa Lipase using Agarose Mercurial Column |
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作者 | 戴達夫; 張元峰; 邱紫文; | 書刊名 | Journal of the Chinese Chemical Society |
卷期 | 49:5 2002.10[民91.10] |
頁次 | 頁957-960 |
分類號 | 361.4 |
關鍵詞 | Lipase; Agarose mercurial column; Kinetic resolution; 2-phenylpropionic acid; |
語文 | 英文(English) |
英文摘要 | Candida rugosa lipase from Sigma was subjected to an agarose mercurial column, and the three fractions thus collected were further desalted using a Sephadex G-25 column. The purification of these isozymes increases their catalytic activities toward the hydrolysis of triacetin. An improved resolution on the hydrolysis of 2-chloroethyl 2-phenylpropionate was also observed by utilizing one of the isolated isozymes CRL II. |
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