頁籤選單縮合
題名 | Effect of t-Butyl Hydroperoxide on Ca[fec7] Movement in PC12 Pheochromocytoma Cells |
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作者姓名(外文) | Lu,Cheng-hsien; Su,Warren; Lo,Yuk-keung; Chen,Wei-chung; Chang,Wen-neng; Wang,Jue-long; Tsai,Yu-chun; Lee,Ping-yu; Jan,Chung-ren; | 書刊名 | 中國生理學雜誌 |
卷期 | 45:2 2002.06[民91.06] |
頁次 | 頁51-56 |
分類號 | 364.64 |
關鍵詞 | Ca[fec7]; Ca[fec7] stores; Fura-2; t-butyl hydroperoxide; PC12 cells; Thapsigargin; |
語文 | 英文(English) |
英文摘要 | The effect of the oxidant t-butyl hydroperoxide on intracellular free levels of Ca²⁺ ([Ca²⁺]i) in PC12 pheochromocytoma cells was examined by using fura-2 as a fluorescent dye. t-Butyl hydroperoxide induced an increase in [Ca²⁺]i, in a concentration-dependent fashion between 50-250 μM with an ECS0 of 100 μM. The [Ca²⁺]i signal consisted of a slow rise and a sustained phase. The response was decreased by 65% by removal of extracellular Ca²⁺. In Ca²⁺-free medium, pretreatment with 1 μM thapsigargin (an endoplasmic reticulum Ca²⁺ pump inhibitor) abolished 150 μM t-butyl hydroperoxide-induced [Ca²⁺]i, increase, and conversely, pretreatment with t-butyl hydroperoxide abrogated thapsigargininduced [Ca²⁺]i increase. The 150 μM t-butyl hydroperoxide-induced [Ca²⁺]i, increase in Ca²⁺ medium was reduced by 42±5% by pretreatment with 0.1 μM nicardipine but not by 10 μM verapamil, nifedipine, nimodipine or diltiazem, or by 50 μM La³⁺ or Ni²⁺. Pretreatment with 10 μM t-butyl hydroperoxide for 40 min did not affect 10 μM ATP-induced [Ca²⁺]i increase. Together, the results show that t-butyl hydroperoxide induced significant [Ca²⁺]i increase in PC12 cells by causing store Ca²⁺ release from the thapsigargin-sensitive endoplasmic reticulum pool in an inositol 1,4,5-trisphosphate-independent manner and by inducing Ca²⁺ influx via a nicardipine-sensitive pathway. |
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