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題名 | 紫背與綠背一點癀無菌繁殖之研究=In Vitro Propagation of Nervilia purpurea (Hay.) Schetr. and N. ragoana Gaud. |
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作者 | 陳振義; 葉茂生; Chen, Jenn-yih; Yeh, Mau-shing; |
期刊 | 農林學報 |
出版日期 | 20020600 |
卷期 | 51:2 2002.06[民91.06] |
頁次 | 頁21-32 |
分類號 | 435.431 |
語文 | chi |
關鍵詞 | 一點癀; 無菌繁殖; 再生; 根莖; 球莖; Nervilia purpurea; Nervilia aragoana; Tissue culture; Propagation; Rhizome; Induction; |
中文摘要 | 本研究以採自屏東恆券之紫背與綠背一點?為材料,利用植株之根堅為培植體,探 討適合本株再生及繁殖之條件,以建立其無菌繁殖技糐,作為大量增殖之用,其結果如下: 紫背與綠背一點?根堅增殖以添加0.5mg/l BA及1mg/l NAA之MS培養基效果最佳。經 90天後紫背一點?瓶底平均可誘導20個球堅,將這些球莖種植,在細與泥碳土(1:1)之介質, 可直接長成植株;而綠背一點?培養過程中並沒有球莖產生,但再以黑殕培養30天後平圾可 誘導誘導形成9個球莖。 綠背一點?繁殖之根莖,以MS培養基添加1.5g/l peptone及1.5g/l活性碳之培養基,培 養90天,平均每瓶可直托誘導26株小植株,時間只要6-8個月,經二週健化移植至細沙與 泥碳土之介質栽培,可長成正常植株。 |
英文摘要 | The purpose of this study was to investigate the rhizome to find out a suitable cultural medium for N. purpurea and N. aragoana, which origined from Pyng Dong. Further, we try to explore the best condition for plantlet regeneration through tissure culture, in order to establish a quickly propagation technique. The results were shown as following: The best medium of rhizome propagation for N. purpurea and N. aragoana was MS supplemented with 0.5mg/lBA and 1mg/l NAA(Code BN0.5-1). After 90 days culture, corm could be induced and become plantlet after sowing for N. purpurea. However, no corm induction was observed for N. aragoana. Plantlet could be induced from rhizome of N. aragoana through MS supplemented with 1.5g/l peptone and 1.5g/l activated charcoal, an required 6-8 months. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。