頁籤選單縮合
題 名 | Neural and Cardiac Activities Are Altered by Injection of Picomoles of Glutamate into the Nucleus Ambiguus of the Rat |
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作 者 | Shaw,Fu-zen; Yen,Chen-tung; Chen,Ruei-feng; | 書刊名 | 中國生理學雜誌 |
卷 期 | 45:2 2002.06[民91.06] |
頁 次 | 頁57-62 |
分類號 | 398.2 |
關鍵詞 | Microinjection; Depolarizing block; Nucleus ambiguus; Heart rate; Single-unit recording; |
語 文 | 英文(English) |
英文摘要 | A quantitative evaluation of the thresholds of changes in the firing rate/pattern and depolarizing block of the neuron and the bradycardiac response by pressure microinjection of 10 mM glutamate (Glu) into the region of the nucleus ambiguus (NA) of the ventral medulla was performed in anesthetized rats. A change in neuronal activity was shown with injection of about 2 pmol of Glu. A depolarizing block of single-unit activity could be observed at 2.9±0.3 nl (~30 pmol, n=22). Maximal bradycardiac response (~50±5%) was elicited with 4.4±0.7 nl (~50 pmol, n=10), which is significantly smaller than the ranges used in previous studies. Based on these results, a safe and effective use of 10 mM Glu to induce neuronal or physiological response should be in the range of a few nanoliters and less than 100 pmol, especially for the NA. |
本系統中英文摘要資訊取自各篇刊載內容。