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題 名 | 臺灣山蘇花葉原體組織培養之研究=In-Vitro Culture of Leaf Primordium of Asplenium nidus |
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作 者 | 全中和; | 書刊名 | 花蓮區農業改良場研究彙報 |
卷 期 | 17 1999.12[民88.12] |
頁 次 | 頁53-63 |
分類號 | 435.43 |
關鍵詞 | 臺灣山蘇花; 葉原體; Asplenium nidus; Leaf primordium; |
語 文 | 中文(Chinese) |
中文摘要 | 台灣山蘇花葉原體培養在不含生長素的MS半固體培養基上培養,三個月後可清楚看見由培植體表面長出類似癒合組織之凸起物,將這些凸起物分切數塊放入1/2MS液體培養基內懸浮培養二週,可形成兩倍的癒合組織,取這些癒合組織放入添加Benzyl adenine (BA)0.2∼5mg/l的MS半固體培養基上培養,可形成大量芽體和癒合組織。若將葉原體直接培養在添加BA 5mg/l的MS半固體培養基上,則三個月後可清楚的看見由培植體表面形成多個芽原體,經過分切之後再放入1/2MS液體培養基內懸浮培養二週後,可形成更多類似芽原體和癒合組織,將這些組織繼代至含BA 0.2∼5mg/l的MS或1/2 MS半固體培養基上培養則會形成叢生芽體和多數癒合組織。芽體的形成以1/2MS培養基較全量MS培養基為佳,而芽體的生長以全量MS培養基較好;芽體形成的數量以添加5mg/l BA的培養基為最多,此外,添加300mg/l酪素水解物(casein hydrolysate)有助於芽體的生長。 |
英文摘要 | The callus-like obstrusion had been formed from the surface of the leaf primordium of Asplenium nidus in the regulator-free MS medium after three-month cultivation. The reformed obstrusion would grow as double as the old one, when it was cut into many pieces and was put into 1/2 MS liquid medium with two-week suspension culture. Then these calli were put into the MS semi-solid medium supplimented with 0.2-5 mg/l BA. The shoot primordium would be formed directly from the surface of the explant after three-month cultivation in the MS semi-solid medium supplimented with 5 mg/l BA. The new shoot primordia and calli would be formed and grown more and more, if the bud primordum was cut into several pieces and was cultured within the 1/2 MS suspension medium about two weeks. Multiple adventitious buds and calli had been formed after cultivating these tissues in the MS or 1/2 MS semi-solid medium supplimented with 0.2-5 mg/l BA. The shoots formed in the 1/2 MS semi-solid medium were better than in the MS medium, however shoots grown in the MS medium were better than in the 1/2 MS semi-solid medium. The amount of shoots would be greatly formed in the MS medium supplimented with 5 mg/l BA. Furthermore, it sould be much better for the shoots to grow in the medium supplimented with 250 mg/l of casein hydrolysate. |
本系統中英文摘要資訊取自各篇刊載內容。