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頁籤選單縮合
題 名 | Molecular Cloning and Sequencing of Light Chain cDNA from a Zucchini Yellow Mosaic Virus-Specific Monoclonal Antibody=矮南瓜黃化嵌紋病毒單元抗體輕鏈基因之分子選殖與核酸序列分析 |
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作 者 | 王惠亮; | 書刊名 | 植物保護學會會刊 |
卷 期 | 42:3 2000.09[民89.09] |
頁 次 | 頁183-194 |
分類號 | 435.262 |
關鍵詞 | 分子選殖; 矮南瓜黃化嵌紋病毒; 融合瘤; Molecular cloning; Zucchini yellow mosaic virus; Hybridoma; |
語 文 | 英文(English) |
中文摘要 | 矮南瓜黃化嵌紋病毒鞘蛋白質單元抗體F28 3F1C4B2,為一具IgG1 重鏈和kappa輕鏈之單元抗體。由產生單元抗體之融合瘤細胞萃取獲得單元抗體之訊息核酸(mRNA),並建立此單元抗體基因之互補去氧核醣核酸(cDNAs)。利用對單元抗體kappa輕鏈基因固定區(constant region)核��酸序列具專一性之寡核��酸探針,成功選殖到此單元抗體kappa輕鏈基因之全長度互補去氧核醣核酸(983 bp)。此互補去氧核醣核酸之核��酸序列分析結果顯示,序列具有一個33 bp (1-33) 之5'端非轉譯區;一個702 bp (34-735) 之開放轉譯架構(open reading frame),其中包含60 bp (34-93) 轉譯成20個胺基酸標的蛋白質序列(leader peptide sequence),318 bp (94-411) 變異區(variable region)序列,和324 bp (412-735) 固定區序列。kappa輕鏈基因之互補去氧核醣核酸之核��酸序列3' 端具有一個208 bp (736-943) 之非轉譯區(nontranslated region)序列,其中包含一個AATAAA序列 (921-926) 位於連結3' 端poly A 前23個核��酸位置和一個poly A 尾區序列。 |
英文摘要 | A cDNA library was constructed from mRNAs isolated from a hybridoma cell line, F28 3F1C4B2, producing monoclonal antibody specific to the coat protrin of zucchini yellow mosaic virus (ZYMV). This hybridoma cel was determined to be a secretor of IgGl heavy chain and kappa light chain class. A full-length light chain cDNA (983 bp), Kappa-1144(K-1144), was selected by hybridization with a specific oligonucleotide probe. The sequence of K-1144 cDNA clone contained a 5' untranslated region (33 bp, 1-33), an open reading frame (702 bp, 34-735) including a 20 amino acid leader peptide sequence (60 bp, 34-93), a variable region (318 bp, 94-411) and c constant region (324 bp, 412-735). The sequence of the 3' end of this clone contained the untanslated region (208 bp, 736-943) with a prototype sequence of AATAAA (921-926) at a position 23 nucleotides before the poly A region, and a poly A region (944-983). |
本系統中英文摘要資訊取自各篇刊載內容。