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題名 | 日月穴與膽囊的解剖生理關係: 一個使用辣根過氧化物酵素法的研究=Anatomicophysiological Relationship of RIYUE ACUPOINT and Gallbladder: A Study Using Horseradish Peroxidase Method |
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作者 | 謝慶良; 曾昌衍; 姥芝瑞; 謝慶竇; 林昭庚; Hsieh, Ching-liang; Tseng, Chang-yean; Lao, Chih-jui; Hsieh, Ching-tou; Lin, Jaung-geng; |
期刊 | 中國醫藥學院雜誌 |
出版日期 | 19981200 |
卷期 | 7:4 1998.12[民87.12] |
頁次 | 頁25-30 |
分類號 | 414.5 |
語文 | chi |
關鍵詞 | 日月穴; 膽囊; 辣根過氧化物酵素; 二氨基聯苯胺; 胸脊神經節; Riyue acupoint; Gallbladder; Horsseradish peroxidase; HRP; DAB(3-3' diamino benzendine); Thoracic spinal ganglia; |
中文摘要 | 日月穴自古以來被用來治療肝膽經之病症,中醫認為日月穴和膽囊有著密切的關 係,但它們在解剖生理學上的關係到今仍然不十分清楚。因此本研究首先利用人類成人的屍 體來觀察日月穴與膽囊在解剖上的關係, 然後使用辣根過氧化物酵素( Horseradish peroxidase,HRP )分別從天竺鼠的日月穴和膽囊注射,然後顯微觀察它們的傳入神經元在 胸部脊髓神經節的分布。本研究分成人體的觀察和動物的觀察兩部份:一)我們將十具人類 的屍體,依照同身寸取穴法將兩側的日月穴定位,然後在穴位處做分層的解剖觀察其組織特 徵,最後打開腹腔觀察日月穴和膽囊的關係; 二)我們將 30 隻天竺鼠分成三組如下:1 ) 對照組( 5 隻): 沒有施行 HRP 注射; 2 )日月穴組( 15 隻):在右側日月穴施行 HRP 注射;3 )膽囊組( 10 隻):在膽囊施行 HRP 注射。 在 HRP 注射 48-72 小時後, 我們將這些動物犧牲取出胸部右側脊髓神經節,然後使用二氨基聯苯胺呈色後做顯微觀察日 月穴和膽囊之傳入神經元在脊髓神經節之分布。結果發現人體的日月穴在解剖上並沒直接與 膽囊相連接。日月穴傳入神經元分布在第四到第八胸部脊髓神經節,而膽囊的傳入神經元是 分布在第三到第八胸脊神經節,兩者之間五個胸部脊髓神經節有著重疊的關係存在。 我們的結論是日月穴和膽囊在實質是沒有直接的連接,但兩者之間對於感覺訊息的傳入 有著密切的連繫關係存在,如此說明針刺日月穴可以治療肝膽經的疾病,這些結果給傳統中 醫針灸典籍所記載的日月穴為膽的募穴提出有利的佐證。 |
英文摘要 | The Riyue acupoint has been used to treat hepato-biliary disorders for a long time. The Riyue acupoint has been cosnidered to be closely related to gallbladder by Chinese medical doctors, but the anatomicophysiological relationship between them still remains unclear. Therefore, first, we observed the anatomical connection between Riyue acupoint and gallbladder in adult human cadavers. Then, we injected Horseradish peroxidase (HRP) into Riyue acupoints and gallbladder of guinea pigs for further observing microscopically the distribution of their respective afferent neurons in the thoracic spinal ganglia. The experiment was divided into human cadavers observation and animal observation as follows: 1) we located the Riyue acupoints bilaterally on ten human cadavers by the porortional unit measurements, then, we dissected the tissues layer by layer and observed their individual characterics. Finally, we explored the peritoneal cdavity to observe the relationship of Riyue acupoint and gallbladder; 2) a total of 30 guinea pigs were divided into three groups as follows; a) contrast group (n=5): without HRP injection: b) Riyue acupoint group (n=15): HRP injection into right Riyue acupoint; c) gallbladder group (n=10): HRP injection into gallbladder. 48-72 hours after HRP injection, they were sacrificed and their right thoracic spinal ganglia were removed. The specimens were then stained by DAB (3-3' diamino benzendine) for microscopic observations on the distribution of afferent neurons. Our results showed that the Riyue acupoints did not directly connect with gallbladders in human. The afferent neurons from the Riyue acupoints distributed on the fourth to eighth thoracic spinal ganglia, while those from gallbladders distributed on the third to eighth thoracic spinal ganglia. Therefore, overlaping in the five spinal ganglia between from Riyue acupoint and from gallbladder afferent sensory neruons. Our conclusions are that there are no direct essential connection between Riyue acupoint and gallbladder, but close relationships between their afferent sensory informatory connection. Therefore, we might explain the reason by the acupuncture stimulation of Riyue acupoint could be used to treat the hepato-biliary diseases. Moreover, our results could also elucidate the cause that the Riyue acupoint was traditionally defined as the alarm point of gallbladder meridian by ancient Chinese medical books about acupunture. |
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