頁籤選單縮合
| 題 名 | Inhibitory Effect of Evodiamine on Aldosterone Release by Zona Glomerulosa Cells in Male Rats |
|---|---|
| 作 者 | Hung,Pei-hsuan; Lin,Lie-chwen; Wang,Geui-jane; Chen,Chieh-fu; Wang,Paulus S.; | 書刊名 | 中國生理學雜誌 |
| 卷 期 | 44:2 2001.06[民90.06] |
| 頁 次 | 頁53-57 |
| 分類號 | 414.52 |
| 關鍵詞 | Evodiamine; Zona glomerulosa cells; Aldosterone; 11β-hydroxylase; |
| 語 文 | 英文(English) |
| 英文摘要 | Evodiamine is a bioactive alkaloid extracted from a Chinese herb named Wu-Chu-Yu, which possesses thermoregulatory, analgesic, and cardiovascular effects. Some studies have demonstrated that evodiamine reduces blood pressure through acting on endothelium and smooth muscle cells to produce a vasodilatory effect, but whether it affects aldosterone secretion is unclear. The purpose of this study was to examine the effect of evodiamine on aldosterone release in adrenal zona glomerulosa (ZG) cells. ZG cells were isolated from the adrenal glands of adult male rats and incubated with angiotensin II (Ang II, 1x10^(-7)M) and ³H-pregnenolone in the presence or absence of evodiamine 1×10^(-6)~l×l0^(-3) M) at 37°C for one hour. The concentration of aldosterone in the media was measured by a radioimmunoassay. The level of radioactivity incorporated into aldosterone and its precursors after incubation of ZG cells with ³H-pregnenolone was analyzed by thin-layer chromatography. The results demonstrated that evodiamine decreased the basal level of and Ang H-induced release level of aldosterone in rat ZG cells. Administration of evodiamine also decreased the level of radioactivity incorporated into ³H-corticosterone and ³H-aldosterone following incubation of ZG cells with ³H-pregnenolone. This suggest that evodiamine affects aldosterone release in rat adrenal glomerulosa cells by acting on Ang TI-associated pathway and reducing the activity of β-hydroxylase (an enzyme which coverts deoxycorticosterone to corticosterone) during the steroidogenesis of aldosterone. |
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