頁籤選單縮合
| 題 名 | The HPLC Resolution of Nucleic-Acid Bases and Analog Hypoxanthine on R,S-2-hydroxypropyl Derivatized β-Cyclodextrin Bonded Chiral Stationary Phase Using a Water-Based Mobile Phase |
|---|---|
| 作 者 | 陳世晞; | 書刊名 | Journal of the Chinese Chemical Society |
| 卷 期 | 48:4 2001.08[民90.08] |
| 頁 次 | 頁751-756 |
| 分類號 | 340 |
| 關鍵詞 | Nucleic-acid bases; Cyclodextrin; |
| 語 文 | 英文(English) |
| 英文摘要 | A HPLC approach using R,S-2-hydroxypropyl derivatized b-cyclodextrin packed column as the stationary phase was developed to resolve five nucleic-acid bases and analog hypoxanthine in the reversed-phase mode. These bases are not only similar in structure but also very close in basicity. However, the resolution can be completed in less than ten minutes and is considered to be better carried out on the R,S-2-hydroxypropyl derivatized b-cyclodextrin phase than that obtained on the native b-cyclodextrin phase under the same chromatographic conditions. The mechanism involved in the resolution is believed to be inclusion complexation between the analyte and the cavity of cyclodextrin in the reversed-phase mode. The retention time was found relevant to the size of the analyte. The number of groups on analyte that is available to form hydrogen bonding with hydroxyl groups on CDs also affects the retention scale. Factors of introducing organic acid and base or organic modifier such as methanol to the water-based mobile phase or increasing their percentages in the mobile phase decreases the retention time without degrading the resolution significantly. |
本系統中英文摘要資訊取自各篇刊載內容。