頁籤選單縮合
題名 | Mucin Gene Expression in Gallbladder Epithelium with Black Pigment Stone Ascertained by in Situ Hybridization=含黑色膽石之膽囊上皮細胞之粘液基因排列 |
---|---|
作者 | 李金德; 劉燦鑫; Lee, King-teh; Liu, Tsan-shium; |
期刊 | The Kaohsiung Journal of Medical Sciences |
出版日期 | 20011000 |
卷期 | 17:10 2001.10[民90.10] |
頁次 | 頁517-523 |
分類號 | 415.456 |
語文 | eng |
關鍵詞 | 黑色膽石; 膽囊上皮細胞; 粘液基因; Mucin; Gene expression; Gallbladder; Black pigment stone; |
中文摘要 | 黑色膽石成因異於膽色素結石或膽固醇結石,它主要形成於肝硬化或 溶血性病人的膽囊內。一般認為膽石症的形成與膽汁內的粘液醣蛋白有關,而粘 液醣蛋白主要來自膽上皮細胞,因此本研究亟想探討含黑色膽石的膽囊上皮細 胞,其膽囊上皮細胞之粘液醣蛋白的基因排列與正常不含結石的膽上皮細胞有何 差異。我們以含黑色結石之膽囊上皮10例為研究對象,並以6位正常膽囊為對 照組,以in situ hybridization的方法對MUC1-4,MUC5B及MUC6的探測,結果 我們發現(1)所有的黑色結石的膽囊上皮粘液素皆含有MUC1,MUC3,MUC5B及 MUC6,而正常膽囊相對的僅有33.3%,83.3%及66.7%的膽囊上皮含有該類基因 排列,亦即黑色結石的膽囊上皮粘液的表現增加(2)MUC2及MUC4未在正常膽 囊上皮出現,卻出現於20%及60%的黑色結石膽囊上皮,因此我們認為含黑色 結石的膽囊上皮細胞,其粘液基因的表現確實異於正常的膽囊上皮細胞;而黑色 結石膽囊上皮的粘液基因的表現,以MUC2,MUC5B及MUC6最明顯,這些粘 液素對結石的形成有相當大的關係。 |
英文摘要 | Black pigment stones are usually found in patients with liver cirrhosis or hemolytic disease. Mucoglycoproteins are present in a significant amount in black pigment stones and contribute to the matrix of gallstones. Epithelium of stone-containing gallbladders contains much more mucin than those without stones. In this study, we try to determine by in situ hybridization the mucin gene expression in black stone-containing gallbladders and try to find the diversity of mucin gene expression in gallbladders containing black pigment stones and those without stones. In situ hybridization with DIG-tailed oligonucleotides was performed on sections of paraffin-embedded tissues of gallbladders with black pigment stones (n=10) and those without stones (n=6) to identify the expression of MUC1, MUC2, MUC3, MUC4, MUC5B and MUC6 in gallbladder epithelium. The findings showed that (1) mRNA expression of MUC1, MUC3, MUC5B and MUC6 were found in all gallbladders with black pigment stones, while they were expressed in 33.3%, 83.3%, 83.3% and 66.7% respectively in those without stones. They were expressed more strongly and extensively in gallbladders with stones when compared to those without stones. (2) MUC2 and MUC4 labeling were absent in gallbladders without stones, while they were present in 20% and 60% of gallbladders with black pigment stones, respectively. We conclude that MUC3, MUC5B and MUC6 were the main mucin gene expression in either gallbladder with or without stones. Altered mucin gene expression occurred in gallbladders with black pigment stones, such as the presence of MUC2 and MUC4 and increased expression of MUC1, MUC3, MUC5B and MUC6 in black stone-containing gallbladders. The higher incidence and stronger labeling intensity of mucin gene expression of MUC2, MUC3, MUC5B and MUC6 in black stone-containing gallbladder may reflect abundant mucin content in these gallbladders. Increased expression of MUC2 and MUC4 in black stone-containing gallbladder epithelium indicated that intestinal metaplasia and altered mucin genes could occur in diseased gallbladders. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。