頁籤選單縮合
題名 | Fendiline-Induced Ca[fec7] Movement in A10 Smooth Muscle Cells |
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作者姓名(外文) | Lo,Yuk-keung; Cheng,Jin-shiung; Wang,Jue-long; Lee,Kam-chung; Chou,Kang-ju; Chang,Hong-tai; Tang,Kwong-yui; Jan,Chung-ren; | 書刊名 | 中國生理學雜誌 |
卷期 | 44:1 2001.03[民90.03] |
頁次 | 頁19-24 |
分類號 | 418.22 |
關鍵詞 | A10 cells; Fendiline; Fura-2; Ca[fec7] signaling; Smooth muscle; |
語文 | 英文(English) |
英文摘要 | The effect of fendiline, an anti-anginal drug, on cytosolic free Ca²⁺ levels ([Ca²⁺])i A10 smooth muscle cells was explored by using fura-2 as a Ca²⁺ indicator. Fendiline at concentrations between 10- 50µM increased [Ca²⁺]i in a concentration-dependent manner with an EC5O of 20 µM. External Ca²⁺ removal reduced the Ca²⁺ signal by 75%. Addition of 3µM Ca²⁺ increased [Ca²⁺]i in cells pretreated with fendiline in Ca²⁺-free medium. The 50 µM fendiline-induced [Ca²⁺]i increase in Ca²⁺-containing medium was inhibited by 10 µM of La³⁺, nifedipine, or verapamil. In Ca²⁺-free medium, pretreatment with 1 µM thapsigargin (an endoplasmic reticulum Ca²⁺ pump inhibitor) to deplete the endoplasmic reticulum Ca²⁺ store partly inhibited 50 µM fendiline-induced Ca²⁺ release; whereas pretreatment with 50 µM fendiline abolished 1 µM thapsigargin-induced Ca²⁺ release. Inhibition of phospholipase C activity with 2 µM U73122 did not alter 50µM fendiline-induced Ca²⁺ release. Incubation with 50 µM fendiline for 10-30 min decreased cell viability by 10-20%. Together, the findings indicate that in smooth muscle cells fendiline induced [Ca²⁺]i, increases. Fendiline acted by activating Ca²⁺ influx via L-type Ca²⁺ channels, and by releasing internal Ca²⁺ in a phospholipase C-independent manner. Prolonged exposure of cells to fendiline induced cell death. |
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