頁籤選單縮合
題 名 | Measurement of Intracellular Ion Dynamics with Microfluorescent Ration Imaging System |
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作 者 | Lin,Chii-wann; Yao,Chih-jung; Ko,Te-son; Lin-shiau,Shoei-yn; | 書刊名 | 醫學工程 |
卷 期 | 10:3 1998.06[民87.06] |
頁 次 | 頁13-20 |
分類號 | 410.1644 |
關鍵詞 | Ratio imaging system; Fluorescent image; Calcium; Intracellular pH; Fura-2; BCECF; Cell culture; |
語 文 | 英文(English) |
英文摘要 | To study the ionic dynamics of spatial distribution and cellular signaling, we have setup a microfluorescent ratio imaging system for quantitative measurement of intracellular calcium and proton concentration with fluorescent indicator, Fura-2 and BCECF. Combined with cell culture techniques, this setup can measure responses from single cell and cell to cell interactions in time lapse mode. The in-homogeneous distribution of intracellular calcium ions under resting condition and after chemical stimulus can also be clearly seen and subjected to quantitative measurement. The results indicate that the resting level of calcium is around 90 nM before maturation (2 DIV.) while significantly raised to around 110 nM after maturation (7 DIV.) in cultured cerebellar granule neurons. Thapsigargin (Thsg) does not transient increase intracellular calcium level as reported in other cell preparations. |
本系統中英文摘要資訊取自各篇刊載內容。