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題 名 | Characterization of 1-aminocyclopropane-1-carboxylate (ACC) Oxidase in Broccoli Florets and from Escherichia coli Cells Transformed with cDNA of Broccoli ACC Oxidase=青花菜之花芽及轉型到大腸菌的ACC氧化酶之定性 |
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作 者 | Kasai,Yuko; Hyodo,Hiroshi; Ikoma,Yoshinori; Yano,Masamichi; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 39:4 1998.10[民87.10] |
頁 次 | 頁225-230 |
分類號 | 373.8 |
關鍵詞 | 甘藍; 青花菜花芽; 大腸菌; 老化; 轉型者; 1-Aminocyclopropane-1-carboxylate oxidase; ACC oxidase; Brassica oleracea; Broccoli florets; Escherichia coli; Senescence; Transformant; |
語 文 | 英文(English) |
中文摘要 | 在青花菜花芽老化過程中,乙烯形成速率與ACC oxidase活性的快速增加幾乎是平 行的,Cycloheximide顯著抑制ACC oxidase活性的增加,此結果建議ACC oxidase活性的增加 可能導因於新酵素蛋白的合成。從青花菜花芽中萃取之ACC oxidase顯示該酵素活性除了需 要ACC及O�祐@為基質外,尚需亞鐵離子,Ascorbate及CO�砥C從青花菜ACC oxidase cDNA轉 型的大腸菌細胞內分離、純化該重組酵素,發現其與青花菜自然的ACC oxidase在酵素特性 上相似,由SDS-PAGE估算其分子量大約為37 kDa,此純化的重組酵素於兔子體內進行多株抗 體的生成,結果發現此多株抗體與青花菜花芽內的ACC oxidase有交互反應,西方墨點分析 顯示該酵素蛋白量在整個老化漸進過程中幾乎相似。 |
英文摘要 | During senescence of broccoli (Brassica oleracea L. var. italica) florets (flower buds), the rate of ethylene production increased, almost paralleling the rapid increase in 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity. The increase in ACC oxidase activity was strongly suppressed by the administration of cycloheximide, suggesting that it may have resulted from the de novo synthesis of enzyme protein. ACC oxidase extracted from broccoli florets exhibited the requirements for ferrous ion, ascorbate, and CO�疳n its activity besides ACC and O�畝s substrates. ACC oxidase was isolated and purified from Escherichia coli cells transformed with cDNA of broccoli ACC oxidase. The recombinant bacterial enzyme was very similar to that of native broccoli enzyme in its enzymatic properties. The molecular mass of the purified enzyme from the transformed cells was estimated to be 37 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The polyclonal antibody was raised in a rabbit against the purifed enzyme isolated from the transformed bacteria, which was found to cross react with the enzyme of broccoli florets. Western blot analysis showed that ACC oxidase protein was detected at an almost similar level over the progressive stages of senescence. |
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