頁籤選單縮合
題 名 | Chaperone Function of Rat Lens α-Crystallin |
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作 者 | 何愈; 許銘清; 黃福永; | 書刊名 | Journal of the Chinese Chemical Society |
卷 期 | 45:2 1998.04[民87.04] |
頁 次 | 頁285-292 |
分類號 | 340 |
關鍵詞 | α-crystallin; Chaperone; Hydrophobic interaction; |
語 文 | 英文(English) |
英文摘要 | A solution of γ-crystallin became turbid upon heating at 65℃ for 30 minutes; however, addition of α-crystallin suppressed this thermal aggregation. It was found the effective chdperone function could be achieved with the molar ratio of α/γ greater than 1/20. In terms of crystallin subunit, five molecular α-crystallin subunits could afford chaperone for one molecular γ-crystallin. The gel filtration profile of the sample solution, containing α- and γ-crystallins and preincubation at 65 ℃ for 30 minutes, showed complex formation between α- and γ-crystallins, indicating α-crystallin was bound to thermally denatured γ-crystallin. A 1- anilinonaphthalene-8-sulfonic acid (ANS) fluorescence study showed that α- crystallin has more hydrophobic regions exposed after thermal incubation. In the presence of urea, both the α-crystallin chaperone activity and the ANS fluorescence intensity decreased. Accordingly, hydrophobic regions of α-crystallin play an indispensible role in its chaperone activity. |
本系統中英文摘要資訊取自各篇刊載內容。