頁籤選單縮合
題 名 | Identification of Anti-Golgi Antibody by Double-Staining Method with Antibodies to β-COP=以雙染色法利用抗β-COP抗體來確認抗高爾基氏體抗體的存在 |
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作 者 | 洪宏翔; 劉紹毅; 莊雅慧; 邱卓凡; | 書刊名 | 中華皮膚科醫學雜誌 |
卷 期 | 16:3 1998.09[民87.09] |
頁 次 | 頁159-163 |
分類號 | 414.8 |
關鍵詞 | 抗高爾基氏體抗體; Anti-golgi antibodies; Anti-β-COP antibodies; Indirect immunofluorescence; |
語 文 | 英文(English) |
中文摘要 | 抗高爾基氏體抗體的相關報告相當少見。在吾人以間接免疫螢光法來篩選自體免 疫性疾病患者的抗核抗體時,我們偵測到抗高爾基氏體抗體。抗高爾基氏體抗體以斑點狀出 現在 Hep-2 細胞核膜的外圍,部份的包圍著核膜並且就正在核膜的外邊。 為了要確認及對抗高爾基氏體抗體作一個定位,我們以雙染色法,利用正常鼠腎細胞為基質 , 並用含抗高爾基氏體抗體的血清及抗β -cop 的抗體來與之作用, β -cop 已知為 nonclathrin-coated 小泡的成份之一並已被接受為高爾基氏體的一個標記。 染色的結果顯 示在核週邊區域都呈現一致的陽性反應。這是一個既新且確實的方法,可用來定位確認抗高 爾基氏體抗體的存在。 |
英文摘要 | Anti-Golgi Antibodies (AGA) were rarely reported. In screening test of anti-nuclear antibody for autoimmune disease patients by indirect immunofluorescence (IIF) using Hep-2 cells as substrate, AGA was detected as a speckled cytoplasmic staining pattern which partially surrounded the nucleus, just outside the nuclear membrance of Hep-2 cells. In order to identify and localize the AGA, we used double-staining method of normal rat kidney (NPK) cells with anti-Golgi antibodies and antibodies to β - COP which is a component of non-clathrin-coated vessicles and recently known to be a good marker for Golgi complex. The result showed identical profile as the positive reaction at perinuclear regions. This is a new and accurate method to identify and localize AGA. |
本系統中英文摘要資訊取自各篇刊載內容。