頁籤選單縮合
題 名 | Further Study of Aldosterone Secretion-Inhibitory Factor and Brain Natriuretic Peptide on Cortisol Production of Guinea Pig Zona Fasciculata Cells |
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作 者 | Fang,Victor S.; Juan,Chi-chang; Won,Justin G. S.; Ho,Low-tone; | 書刊名 | 中國生理學雜誌 |
卷 期 | 43:4 2000.12[民89.12] |
頁 次 | 頁141-147 |
分類號 | 361.44 |
關鍵詞 | ACTH; ACTH receptor; Aldosterone secretion-inhibitory factor; Brain natriuretic peptide; Adrenal zona fasciculata cells; Guinea pig; |
語 文 | 英文(English) |
英文摘要 | The suppressive effect of aldosterone secretion-inhibitory factor (ASIF) and brain natriuretic peptide (BNP-32) on the basal and ACTH-stimulated cortisol production in a primary culture enriched with guinea pig Zona Fasciculata (ZF) cells was further studied. The binding of □I-labeled ACTH(1-24) and ASIF to ZF cells was found to be displaced by ACTH(1-24), [Phe², N1e⁴ and Ala[fed6]]-ACTH(1-24), ASIF, and BNP in a concentration-dependent manner. The binding of □I-labeled [Phe², Nle⁴ and Ala[fed6]]-ACTH(1-24) to two transformed clones of mammalian cells expressing the guinea pig ACTH receptor was also competitively inhibited by ASIF and BNP. ASIF and BNP significantly suppressed ACTH stimulated cAMP production in ZF cells. The 10-and 30-min cellular changes in cAMP induced by ASIF and BNP did not correlate in the rank order with the ultimate magnitude of cortisol suppression observed in ZF cells after a 24-hour treatment with these peptides. Nevertheless, the results did conform to the signaling mechanism of their action. Overall, the findings clearly demonstrated that ASIF and BNP suppressed the adrenocortical function and inhibited ACTH for their antagonistic action against ACTH primarily at the ACTH receptor site. These results support the notion that a physiological role of adrenal medulla in regulating the adrenocortical function may be mediated by the neuropeptides through a paracrine pathway. |
本系統中英文摘要資訊取自各篇刊載內容。