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題 名 | Adenosine Triphosphate Modification of Endotoxin-Induced Otitis Media with Effusion in Gerbils=沙鼠中耳注射三磷酸苷酸對積液性中耳炎的影響 |
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作 者 | 焉本棟; 黃俊生; | 書刊名 | 長庚醫學 |
卷 期 | 21:3 1998.09[民87.09] |
頁 次 | 頁306-311 |
分類號 | 416.821 |
關鍵詞 | 積水性中耳炎; 三磷酸苷酸; 嘌呤接受器; Otitis media with effusion; Adenosine triphosphate; Purinoceptor; |
語 文 | 英文(English) |
中文摘要 | 背景:纖毛運動受損是形成中耳積水主要原因之一,為使纖毛能夠有效清除黏液 (mucus)的膠層(gellayer),需要由離子傳導來調節纖毛液層(periciliary fluid layer) 之深度。若深度太淺,則纖毛會糾結在一起無法驅動雜質;深度超過正常,則纖毛運動無法 有效驅動過量的膠層。三磷酸 酸(ATP)已經在各類組織及細胞中證實有增加離子傳導之效 能。本實驗希望能呈現注射ATP對中耳積水吸收之情形。 方法:(1)動物分組:第一組選取15隻沙鼠,雙耳注入E. coli LPS 100ug/ml,3天後確定有 積水形成後,右耳再注入ATP 10�弸;第二組另選15隻沙鼠,右耳先注入ATP 10�弸,15分鐘 後,雙耳再注入E. coli LPS 100ug/ml。 (2)電子顯微鏡觀察:把動物犧牲後,立刻將耳泡(otic bulla)用2.5% Glutaraldehyde 固定,再進行少掃描式電子顯微鏡處理,以觀察中耳上皮細胞纖毛的情形。 結果:第一組中,15耳接受ATP注入而沒有形成積水者有5例,未注入ATP且積水消失者有 3例;第二組中,15耳接受ATP注入而沒有形成積水者有 5例,沒有注射ATP且積水消失者佔 2例。在25例成功製作的電顯樣本中,發現接受ATP處理者,確實能保有正常的纖毛層,而經 ATP處理者其大致有病理性的變化。 結論:由結果可看出,有無注入ATP對中耳積水之消失與否,並無明顯差異,但是在掃描式 電子顯微鏡之觀察中,可看出不同類型的纖毛變化。沒有注射ATP者,其纖毛變稀少且有嚴 重變形的現象,而有注入ATP者,尚能保存較多的纖毛,與正常組織比對相差無幾。 |
英文摘要 | Background: Impairment of the mucociliary system of the middle ear epithelium is a key step in developing otitis media with effusion. Effective propulsion of the viscoelastic gel layer of mucus requires a control of the depth of the periciliary sol layer by means of epithelial ion transport. In a previous study, we successfully demonstrated modulation of ion transport via short-circuit current changes by specific stimulation of adenosine triphosphate (ATP) receptors in a middle ear cell line. Materials and Methods: Otitis media with effusion was developed in gerbils by infusing Escherichia coli E.coli lipopolysaccaride. In group A, E. coli lipopolysaccharide was first Infused, and after establishment of middle ear fluid, the right ear was infused with 10�弸 ATP, while the left ear remained as the control. In group B, the right ear was infused first with 10�弸 ATP followed by bilateral infusion with endotoxin. The morphology of the middle ear mucosa was studied using scanning electron microscopy (SEM). Results: Group A showed fluid clearance in 5 out of 15 ATP-treated right ears while 3 out of 15 left ears showed clearance. Group B showed persistent presence of fluid in 11 out of 15 ATP-treated right ears, while the left ears showed fluid in 13 out of 15. SEM study consistently demonstrated scanty cilia in the non-ATP treated ears, but in ATP-treated ears the cilia remained abundant. Conclusion: Although the results showed that ATP could not effectively prevent or clear middle ear effusion, the SEM result showed a marked beneficial effect of ATP on preserving ciliary configuration. |
本系統中英文摘要資訊取自各篇刊載內容。