頁籤選單縮合
題 名 | 2,3,5-Triphenyltetrazolium Reduction in the Viability Assay of Ulva fasciata (Chlorophyta) in Response to Salinity Stress=利用2,3,5-Triphenyltetrazolium被還原能力評估裂片石蓴鹽度逆境下之存活能力 |
---|---|
作 者 | 張婉純; 陳敏華; 李澤民; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷 期 | 40:3 1999.07[民88.07] |
頁 次 | 頁207-212 |
分類號 | 379.27 |
關鍵詞 | 鹽度逆境; 存活力; Salinity stress; TTC; Ulva fasciata; Viability; |
語 文 | 英文(English) |
中文摘要 | 本研究利用2,3,5-Triphenyltetrazolium(TTC) 還原為不可溶於水之紅色 formazan 能力評估裂片石蓴 (Ulva fasciata Delile)的。 0.1 克鮮重葉狀體置於 1.5毫升含 TTC 之 5OmM 磷酸鈉緩衝液,還原後之 formazan 以 95% 酒精萃取並在 530nm 下測定吸光值以避免色素干擾。 TTC 還原能力在含 3% NaCl 之 0.8%TTC 溶液培養 18 小時後達最大。 TTC 還原能力在不同大小的裂片石蓴或葉狀體間並無差異,但葉狀體靠附著器之基部(約3公分) 卻明顯較低 (p<0.01) 。以最大葉狀體尖端 2.5 公分片段比較之,全株裂片石蓴在不同鹽度 (5~150%0) 下的 TTC 還原能力在 30% 。達最大,在120%。以上及 5% 。以下最小。淨光合作用及比生長率在不同鹽度 (5~150%0) 下也有相似的趨勢。以30% 。為基礎,相對 TTC 還原能力與相對淨光合作用([] =0.93) 及相對比生長率 ([] =0.90) 有相關。這些結果指出裂片石蓴可在 15~90[]。間存活,而相對 TTC 還原能力可以作為預測耐鹽性的工具。 |
英文摘要 | The salinity tolerance in a marine green macroalga Ulva fasciata was assessed by the reduction of 2,3,5triphenyltetrazolium chloride (TTC) to water-insoluble red formazan. Thallus strips of 0.1 gram fresh weight were incubated in 1.5 ml of 50 mM sodium phosphate buffer (pH 7.4) containing TTC, and the formazan extracted by 95% ethanol was measured at 530 nm to avoid pigment interference. The TTC reduction activity reached the maximal level 18 h after incubation in 0.8% TTC solution in the presence of 3% NaCl. Plants of different sizes had similar TTC reduction activity, but activity in the 3-cm-long base near the rhizoidal portion was lower than in the remaining parts (p<0.01). On exposure of whole plants to various salinities, ranging from 5%o to 150%o, the reduction of TTC in the 2.5 cm tip (the distal end) of the largest thallus peaked in plants grown at 30%o and was low at salinity above 120%o or below 5%o. Both the net photosynthetic 0, evolution rate in the 2.5 cm tip of the largest thallus and the specific growth rate (%/d) had similar trends. Compared to plants grown at 30%0, the relative change in TTC reduction activity is associated with the relative change in specific growth rate (r'=0.90) and also with the relative change in net photosynthetic 0, evolution rate (r2=0.93). These results show that Ulva fasciata survives at salinities ranging from 15%o to 90%0. Tracking relative changes in TTC reduction activity has potential as a tool for predicting salinity tolerance. |
本系統中英文摘要資訊取自各篇刊載內容。