頁籤選單縮合
題 名 | Establishment of a Culture Condition for Improving Short-Term in Vitro Maintenance of Hemocytes from Giant Freshwater Prawn (Macrobrachium rosenbergii)=改善短期維持淡水長腳大蝦(Macrobrachium rosenbergii)血球細胞的體外培養條件 |
---|---|
作 者 | 宋宏紅; 劉怡霞; | 書刊名 | 臺灣水產學會刊 |
卷 期 | 26:2 1999.06[民88.06] |
頁 次 | 頁115-119 |
分類號 | 439.66 |
關鍵詞 | 蝦血球細胞; 短期培養; Prawn hemocyte; Short-term incubation; |
語 文 | 英文(English) |
中文摘要 | 本研究主要針對短期體外維持蝦血球細胞的培養條件進行改善,以利蝦子細胞 性防禦機制的研究。來自淡水長腳大蝦(Macrobranchium rosenbergii)的血球樣本,分別 培養在(1)MCHBSS培養基,於4℃下、30或60分鐘;(2)不同的培養基,於4℃下,30分鐘; 及(3)L-16培養基,於4、25或30℃下,30分鐘。血球培養30分鐘後的活存率(53.3%)高 於培養60分鐘(16.7%)者。使用不同培養基時.培養於L-15(63.5%﹞、L-15胎牛血清(587 %)、及L-15蝦血清(557%)三種培養基的血球活存率無明顯差異;但以MCHBSS培養後,活 存率明顯降低(36.8%)。當血球以l-156培養基於不同溫度下培養30分鐘後,培養在30℃ 的血球活存率(36.1%)明顯低於培養在4℃(56%)或25℃(62.6%)者。 |
英文摘要 | This study intended to improve the short-term in vitro maintenance of hemocytes in order to study cellular defense mechanisms in prawn. Hemocyte samples from Macrobranchium rosenbergii were cultured in (1) modified complete Hank's balanced salt solution (MCHBSS) at 4C for 30 or 60 min, (2) different media at 4 ℃ for 30 minutes, and (3) L-15 medium at 4, 25 or 30℃ for 30 min, respectively. A higher percentage of hemocyte viability was obtained following hemocytes cultured for 30 min (53.3%) compared to cultured for 60 min (16.7%). Using different media, there were no significantly difference of hemocyte viability between L-15 medium (63.5%), L-15-FCS (58.7%) and L- 15-PS (55.7%); however, a significant decrease of viability was obtained following hemocytes cultured in MCHBSS (36.8%). Following hemocytes incubated in L-15 for 30 min at different tempera tures, the viability was significantly lower at 30 ℃ (35.1%) than at 4 ℃ (56.1%) or 25 ℃ (52.6%). |
本系統中英文摘要資訊取自各篇刊載內容。