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題名 | Effects of VEGF and/or VEGF Gene Transfection on Collateral Circulation Development=血管內皮生長因子VEGF121及VEGF165基因轉植對側枝循環發育之影響 |
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作者 | 程兆明; 林繼謨; 黃士銘; 張順浪; 李超群; 蔣連財; 張寶源; | 書刊名 | 臺灣醫學會雜誌 |
卷期 | 99:8 2000.08[民89.08] |
頁次 | 頁603-611 |
分類號 | 415.38 |
關鍵詞 | 血管內皮生長因子; 基因轉植; 側枝循環發育; VEGF; Collateral circulation; Angiogenesis; |
語文 | 英文(English) |
英文摘要 | Background and purpose: Angiogenesis is regulated by various factors, including vascular endothelial growth factor (VEGF). Five isoforms of VEGF have been discovered: VEGF 121 , VEGF 145 , VEGF 165 , VEGF 189 , and VEGF 206 . The teleologic basis for the various VEGF isoforms remains unclear, but different VEGF isoforms may mediate distinct endothelial cell functions such as angiogenesis, vascular permeability, and differen-tiation. We sought to determine the effects of various VEGF isoforms on angiogenesis under ischemic conditions in rabbits. Methods: The effects of VEGF 121 and/or VEGF 165 gene transfection on collateral circulation development in ischemic rabbit hindlimb muscles were investigated by using naked plasmids encoding VEGF 121 or VEGF 165 (pVEGF 121 or pVEGF 165 ), either individually or in combination. pCM���nwas used as the control plasmid. The femoral artery on one side of New Zealand White rabbits was ligated. Ten days later, the ischemic muscles received direct intramuscular injection of pVEGF 121 (500 �瞟), pVEGF 165 (500 �瞟), or pVEGF 121 (250 �瞟) + pVEGF 165 (250 �瞟) in experimental groups, while pCM���n(500 �瞟) was used in the control group. Therapeutic effects were evaluated 30 days later by anatomic and physiologic analysis. Results: Internal iliac angiography showed strong development of collateral circulation in all of the pVEGF-treated groups. In contrast, collateral arteries developed weakly in the control group. Combination treatment with both pVEGF 121 and pVEGF 165 did not result in additional improvement compared with pVEGF 121 or pVEGF 165 treatment alone (angiographic scores: pVEGF 121 = 0.85 �b�n0.10; pVEGF 165 = 0.81 �b�n0.11; pVEGF 121 + pVEGF 165 = 0.83 �b�n0.09; control = 0.53 �b�n0.09; p < 0.01). A favorable response in the development of circulation at the capillary level with pVEGF 121 and/or pVEGF 165 versus pCM���nwas also found. Blood pressure measurement and regional blood flow measurement using colored microspheres revealed similar results. Conclusions: Our results show that direct intramuscular injection of naked DNA encoding VEGF 121 or VEGF 165 , individually or in combination, is an effective method for gene transfer in an animal model of ischemic limbs and results in augmented collateral vascular development and tissue perfusion. |
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