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題 名 | 大白鼠骨性肉瘤細胞的造骨細胞表型之研究=Study of Osteoblastic Phenotypes in Rat Osteosarcoma Cells |
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作 者 | 陳羿貞; 侯連團; 陳坤智; | 書刊名 | 中華牙醫學雜誌 |
卷 期 | 17:2 1998.06[民87.06] |
頁 次 | 頁68-78 |
分類號 | 416.93 |
關鍵詞 | 大白鼠骨性肉瘤細胞; 造骨細胞表型; Rat osteosarcoma cells; Osteoblastic phenotype; |
語 文 | 中文(Chinese) |
中文摘要 | ROS(17/2.8)細胞是一種培養自大白鼠骨性肉瘤組織的分株細胞,本研究以此引進 之細胞株加以培養後,取人類健康牙齦造纖維細胞(fibroblast)為負對照組,利用下列三 種生化試驗法驗證ROS 17/2.8 細胞的造骨細胞特性:(1)以細胞化學染色法觀察培養細胞 的鹼性磷酸�t表現,(2)添加甘油磷酸鹽(��-glycerophosphate)及維生素C於培養液, 檢測上述二種細胞在體外培養環境中的細胞外基質鈣化能力,(3)給予牛副甲狀腺素剌激 後測定前述二種細胞的腺嘌呤核��單磷酸(cyclic adenosine monophosphate, cAMP)之含 量。本研究由上述三方面的試驗結果,充分驗證ROS 17/2.8 細胞的造骨細胞活性之表型, 而牙齦造纖維細胞則無此特性。 |
英文摘要 | Rat osteosarcoma cells (ROS 17/2.8) have been widely used as a cell model for studying cellular metabolism of osteoblasts. However, several characteristics of this cell line such as the expression of markers of osteoblastic differentiation and in vitro mineralization are rarely reported. These factors are important indicators to understand the biological changes associated with the bone remodeling secondary to environmental impact such as mechanical force induced by orthodontic tooth movement. Thus, the purpose of this research was to study these osteoblastic phenotypes in cultured ROS 17/2.8 cells. Human gingival fibroblasts at the 6th passage were used as the negative control in this study. In order to detect the expression of osteoblastic characteristics of ROS 17/2.8 cells, the following investigations were done: (1) cytochemical staining for alkaling phosphatase activity, (2) in vitro mineralization of cultured cells in the presence of ��-glycerophosphate & ascorbic acid, and (3) determining the effect of parathyroid hormone stimulation on cAMP (cyclic adenosine 3',5' -monophosphate) accumulation. Cytochemical staining revealed that ROS 17/2.8 cells were rich in alkaline phosphatase, whereas this enzyme was scarcely expressed in human gingival fibroblasts. ROS 17/2.8 cells grown in the presence of 10 mM β-glycerophosphate and 50μg/ml ascorbic acid formed mineralized nodules, as determined by Von Kossa's and Alizarin red S staining. In contrast, human gingival fibroblasts cultured in similar medium did not form mineralized nodules. The presence of 1.0×10�笐篇/ml bovine parathyroid hormone in culture medium for 5 minutes caused a 5 fold increase in the cAMP level of ROS 17/2.8 cells, while this stimulation was not detected n human gingival fibroblasts. In conclusion, the ROS 17/2.8 cell line expressed several osteoblastic phenotypes in our culture system, implying its validity as a model for the study of bone cell biology. |
本系統中英文摘要資訊取自各篇刊載內容。