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題名 | NPC-15199, A Novel Anti-Inflammatory Agent, Mobilizes Intracellular Ca[feb4]狇in Bladder Female Transitional Carcinoma (BFTC) Cells= |
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作者 | Jan,Chung-ren; Yu,Chia-cheng; Huang,Jong-khing; |
期刊 | 中國生理學雜誌 |
出版日期 | 20000300 |
卷期 | 43:1 2000.03[民89.03] |
頁次 | 頁29-33 |
分類號 | 397 |
語文 | eng |
關鍵詞 | Ca[feb4]狇signaling; BFTC cells; Bladder cell carcinoma; NPC-15199; Fura-2; |
英文摘要 | This report demonstrates that NPC-15199 [(N-(9-fluorenylmethoxycarbonyl)L-leucine)], a novel anti-inflammatory agent, increases intracellular Ca²⁺ concentration ([Ca²⁺]i) in human bladder female transitional cancer (BFTC) cells. Using fura-2 as a Ca²⁺ probe, NPC-15199 (0.1-2mM) was found to increase [Ca²⁺]i concentration-dependently. The response saturated at 2-5mM NPC-15199. The [Ca²⁺]i increase comprised an initial rise, a slow decay, and a plateau. Ca²⁺ removal partly inhibited the Ca²⁺ signals. In Ca²⁺-free medium, pretreatment with 1mM NPC-15199 abolished the [Ca²⁺]i increase induced by 1µM thapsigargin (an endoplasmic reticulum Ca²⁺ pump inhibitor); and after pretreatment with thapsigargin, NPC-15199-induced Ca²⁺ release was dramatically inhibited. This indicates that NPC-15199 released internal Ca²⁺ mostly from the endoplasmic reticulum. Adding 3mM Ca²⁺ increased [Ca²⁺]i in cells pretreated with 1mM NPC-15199 in Ca²⁺-free medium. Together, the findings suggest that in BFTC bladder cancer cells, NPC-15199 induced Ca²⁺ release from the endoplasmic reticulum and activating Ca²⁺ entry. |
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