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題名 | Gel-Filtrated Fractions of Alveolar Bone Extract Contain Factors Promoting Cell Attachment and a Mitogenic Effect on Periodontal Ligament Fibroblasts=齒槽骨萃取物經分餾純化之成份含有促進牙周韌帶纖維母細胞附著和分裂之因子 |
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作者 | 侯連團; 劉謙美; 陳羿貞; 王敏瑩; 陳錦坤; 陳坤智; | 書刊名 | Proceedings of the National Science Council : Part B, Life Science |
卷期 | 24:1 2000.01[民89.01] |
頁次 | 頁33-40 |
分類號 | 416.946 |
關鍵詞 | 齒槽骨萃取物; 牙周韌帶纖維母細胞; 凝膠過濾層析; 細胞附著; Gel filtration; Alveolar bone extract; Periodontal ligament fibroblast; Cell attachment; Mitogenicity; |
語文 | 英文(English) |
英文摘要 | The purpose of this study was to investigate the effect of acetic acid- extracted bone proteins on human periodontal ligament fibroblasts (hPF) with respect to mitogenic and cell attachment promoting activity. Alveolar bone was harvested from healthy donors and subjected to 0.5M acetic acid extraction, dialysis and lyophilization, and gel filtration. Promotion of cell attachment and stimulation of DNA synthesis by the crude extract and gel-filtrated fractions were studied in cultured hPF. Many protein components, varying in molecular weight from 10-14 to 120 kDa, were detectable in 10% SDS-PAGE of the extract. Gel filtration of bone extract disclosed four fractions with molecular weights of 55, 34, 29 and 19-20kDa. Both the 34 and 55kDa fractions at a concentration of 5?/ml, but not the 29- or 19-20kDa fractions, were found to promote cell attachment while only the 55kDa fraction (5?/ml) stimulated DNA synthesis of hPF. Both mitogenic activity and the promotion of the cell attachment by gel-filtrated active fractions were resistant to thermal treatment (70?) and pH (4~8) changes. These findings suggest that acetic acid extract of alveolar bone may contain components which are capable of modulating cell attachment and mitogenesis of hPF. |
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