頁籤選單縮合
題名 | 豬皮膠原蛋白膜與組織之整合性及所誘發之細胞反應=Tissue Integration and Cellular Responses Associated with Porcine Dermal Collagen Membrane |
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作者 | 吳銘芳; 呂炫堃; 李長祐; | 書刊名 | 中華民國獸醫學會雜誌 |
卷期 | 25:4 1999.12[民88.12] |
頁次 | 頁290-296 |
分類號 | 416.9 |
關鍵詞 | 豬皮膠原蛋白膜; 黏連; 組織整合性; 細胞反應; Porcine dermal collagen membrane; PDCM; Adhesion; Tissue integration; Cellular responses; |
語文 | 中文(Chinese) |
英文摘要 | The purposes of this study were to study the cellular responses and tissu integration associated with porcine dermal collagen membrane (PDCM) in vivo. 3% GA-PDCM was implanted in the upper jaw of Wistar rats. The specimens were harvested 1,2,3,5,7,10,14,21, 28, and 42 days after the surgery. One-fifth of the specimens were processed for H&E stain, alcian blue-PAS stain, and the rest of the specimens were frozen immediately and processed for immunohistaochemical stain (ABC method). In order to understand the cellular immunity induced by 3%GA-PDCM, the number of CD4, CD8, CD11b positive cells were counted by using grid screen under light microscope. The results of in vitro study shows that the cultured ginigiva connective tissue presents a good contact with the PDCM and prevented the epithelial cell to move downward. There were more CD4+ cells than CD8+ cells in the local tissue and the ratio between these two cells was 2:1. The counting of CD11b+cells increases following the increasing counting of CD4+ cells. However, the intensity of and the numbers of CD4+, CD8+, CD11b+cells decreased. It indicated that 3%GA-PDCM starts to achieve good tissue integration in connective tissue on day 14. There were no significant pathologic reaction and evidence of tissue damage. In conclusion, these results indicated that PDCM possesses a good quality of tissue integration with adjacent connective tissue. The CD4/CD8/CD11b counting also shows that the immunological response induced by PDCM is a chronic inflammation. |
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