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頁籤選單縮合
題名 | 玉花四季蘭種子低發芽率致因=The Causes of Low Germination Rate of "Cymbidium ensifolium 'Yuh Hwa' in vitro" |
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作者 | 林淑婷; 張正; Lin, Shu-ting; Chang, Chen; |
期刊 | 植物種苗 |
出版日期 | 20120300 |
卷期 | 14:1 2012.03[民101.03] |
頁次 | 頁27-39 |
分類號 | 435.431 |
語文 | chi |
關鍵詞 | 玉花四季蘭; 種子發芽; 種皮; 角質層; Cymbidium ensifolium 'Yuh Hwa'; Seed germination; Integument; Cuticle; |
中文摘要 | 本試驗以人工自花授粉之玉花四季蘭種子進行播種,探討種子發芽困難的原因及改善方法。玉花四季蘭授粉後14週之種子經無菌播種即具有發芽能力,授粉後22週種子發芽率較高,僅為4.2%。授粉後24週後的種子發芽率逐漸下降,授粉28週後的胚大小不再增加。經切片解剖及染色證明授粉後第36週之種子已具有木質化的種皮構造,且胚體外已形成不透水角質層,以次氯酸鈉溶液或超音波震盪處理可破壞外種皮的結構或使種皮與胚分離,並可提高種子發芽率;經TTC染色顯示高達75.9%的胚具有活性,然經種子處理之發芽率僅提高到22.2%。 |
英文摘要 | The handed self-pollination capsules of "Cymbidium ensifolium" 'Yuh Hwa' were used for germination "in vitro" and discussed the reasons about low germination percentage. The seeds of "Cymbidium ensifolium" 'Yuh Hwa' did not get the germination ability until 14 weeks after handed self-pollination. The seeds had the highest germination percentage at 22 weeks after pollination (WAP) of 4.2 %. The embryos of those seeds were developed until 28 WAP. At 36 WAP, the seeds had lignification of the outer integument. The use of sodium hypochlorite solution or ultrasonic wave treatment could structural damage outer integument, and then obtain the highest germination percentage. Seeds germination was restricted by lignified outer integument. Through the test of 2,3,5-tetrazolium triphenyl chloride (TTC), there were 75.9% seeds with vigor embryo, but through aseptic sowing and pre-treatment, only 22.2% seed germinated. Based on the foregoing, some unknown factors effect on the germination will be explored in the future study. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。