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題 名 | Genetic Variation of Recent Infectious Bronchitis Viruses Isolated in Taiwan=臺灣新近分離之雞傳染性支氣管炎病毒之核酸變異分析 |
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作 者 | 張國慧; 蔡向榮; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 25:3 1999.09[民88.09] |
頁 次 | 頁164-170 |
分類號 | 437.717 |
關鍵詞 | 雞傳染性支氣管炎病毒; S1醣蛋白基因; 反轉錄聚合酶連鎖反應; 核酸變異; 核酸序列相似性; Infectious bronchitis virus; S1 glycoprotein gene; Reverse transcriptase polymerase chain reaction; Genetic variation; Sequences similarity; |
語 文 | 英文(English) |
中文摘要 | 研究臺灣雞傳染性支氣管炎病毒(infectious bronchitis virus; IBV)之核酸變異與基因型。將五株1997年臺灣IBV分離株與H120疫苗株應用反轉錄聚合�t連鎖反應(reverse transcription polymerase chain reaction; RT-PCR)與核酸直接定序技術(DNA sequencing)來分析S1醣蛋白基因之C2U-C3L區段(114-341 base)核酸序列,並將定序結果與GenBank上各國已發表的32株IBV進行比對,結果顯示新近分離毒株與H120疫苗株只有51.8%至58.3%之相似性,在六個基因型中與臺灣I群的相似性最高(平均為82.9%),而與臺灣Ⅱ群、麻州群、美國群、歐洲群、荷蘭群的相似性平均介於42.0%-53.4%,故顯示目前在臺灣盛行之IBV以臺灣Ⅰ群之基因型為主,並且與5年前之毒株比較已有相當程度之核酸變異,由此顯示IBV易產生變異,而且臺灣分離株已演化成為本土株。 |
英文摘要 | The genetic variations between five infectious bronchitis virus (IBV) strains isolated from broilers in Taiwan in 1997 and a vaccine strain (H120) were compared by using the reverse transcription-polymerase chain reaction (RT-PCR) and direct sequencing technology. The sequence data for the C2U-C3L region (114-341 base) of S1 glycoprotein gene were also compared with the 32 published reference IBV strains in GenBank. According to the results, the similarities between the H120 vaccine strain and the five Taiwan field strains were only 51.8% to 58.3%. Comparison of the sequences of these strains with genotypes of Taiwan group Ⅱ, Massachusetts, American, European and Dutch group, the similarities were ranged from 42.0% to 53.4%, and it was 82.9% with Taiwan group I genotype. These results indicated that the prevalent IBV strains in Taiwan were closely related to Taiwan group I genotype, and further genetic variations did occur in the field in the past five years. |
本系統中英文摘要資訊取自各篇刊載內容。