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題名 | 常見柴胡中藥材品種之分子鑑定=Molecular Identification of Common Buplerum Species Medicines |
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作者 | 鄭可大; |
期刊 | 中醫藥年報 |
出版日期 | 19990500 |
卷期 | 17:2 1999.05[民88.05] |
頁次 | 頁487-497 |
分類號 | 414.32 |
語文 | chi |
關鍵詞 | 柴胡; 基源; 逢機擴增複製多型性去氧核醣核酸; Bupleurum; Identification; RAPD; |
中文摘要 | 本研究以一般市售的柴胡藥材,如北柴胡、南柴胡、銀柴胡等,臺灣種植的高氏 柴胡及三島柴胡根部之乾品及鮮品及其偽品大葉柴胡為材料,進行逢機擴增複製多型性 DNA 片段 (random amplified polymorphic DNA, RAPD) 的反應,以期建立中藥材分子指紋分析 的圖譜,作為市售中藥材基源鑑定之用。實驗中以六十條引子,每條包含十個任意序列之核 甘酸,對分佈於臺灣北、中、南部之三十家中藥店的市售柴胡,分別進行 RAPD 分析。篩選 適當的引子,所得到的電泳圖譜,將作為今後中藥材基源鑑定比對的依據,同時可提供為中 藥品質管制的參考。 本研究自六十條引子中篩得三條較為適合的引子,這些引子分別為 D1 、D16 及 G18;它們分別在各柴胡品種間顯現多型性,並以此引子對市售三十家中藥店的柴 胡藥材,進行出 RAPD 分析,結果利用一些遺傳標誌,大致可將這些藥材分為三型。此外, 藥材的鮮品及乾品,不同組織部位對 RAPD 分析的結果,差異不大,此結果將減少該技術分 析結果的不穩定性。 |
英文摘要 | In the study, we identified the Chinese drug materials of the species of Bupleurum, for instance, Bupleurum chinense DC., B scorzoneraefolium Wilid., and B. marginatum Wall. ex DC., etc., which sell in the markets, B. falcatum L., and their adulterant, B. Inogiradiatum Turcz., and B. kaoi L.,C.& C., which plant in the field in Taiwan, with RAPD analysis. In the experiments, sixty lOrner arbitrary primers were used to screen the above samples, and to the crude drugs which sold by thirty randomly selected stores in Taiwan, for obtaining available primers which clearly differentiate among them. The aims of the present study are to construct the identification model of molecular biotechniques applying on Chinese drugs, Total DNA isolated from the samples were used as templates in RAPD analysis using thirty random decamer primers, respectively. Three of the primers, OPD-OI, OPD-16, and OPG-18, revealed reproducibly distinct RAPD profiles. We found there were three RAPD fingerprinting patterns of OPD-OI and OPD-16, respectively, from thirty stored Bupleurum samples. In addition, the results of RAPD were not different between the dried and fresh samples, not among the leaf, stem and root of single individual. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。