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相關文獻
- Detection of Fetal Cells with Common Chromosomal Aneuploidies in Maternal Blood
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題 名 | Detection of Fetal Cells with Common Chromosomal Aneuploidies in Maternal Blood=以母親血液偵測常見胎兒染色體異常 |
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作 者 | 郭保麟; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 98:6 1999.06[民88.06] |
頁 次 | 頁433-439 |
分類號 | 417.133 |
關鍵詞 | 母親; 血液; 胎兒; 染色體異常; Magnetic-activated cell sorting; Fluorescence in situ hybridization; Noninvasive diagnosis; Aneuploidy; Prenatal diagnosis; |
語 文 | 英文(English) |
英文摘要 | This study was designed to address the feasibility of detection of fetal cells with chromosome abnormalities in maternal blood. Peripheral venous blood samples were collected from 150 pregnant women 1 day to 8 weeks before invasive examinations (amniocentesis, chorionic villus sampling, or fetal blood sampling). Fetal nucleated red blood cells were isolated by using a triple-density gradient followed by magnetic-activated cell sorting to select CD71 cells. Fluorescence in Situ hybridization (FISH) with probes specific for chromosomes X, Y, 13, 18, and 21 was used to detect fetal cells from aneuploid pregnancies. The hybridization efficiency was greater than 98% for each probe in normal controls, and approximately 20% all cells failed to hybridize after magnetic-activated cell sorting. Of the 10 aneuploid pregnancies identified with invasive procedures, trisomic fetal cells were identified in eight. The frequency of fetal cells in the sorted specimens ranged from 0 to 223 per 10,000 maternal nucleated cells. Although the aneuploid fetal cells could be successfully detected in eight of 10 patients in the current study, the existence of some limiting factors, such as scarcity of fetal cells and poor hybridization efficiency of FISH, raises questions about its clinical suitability for routine use, |
本系統中英文摘要資訊取自各篇刊載內容。