查詢結果分析
來源資料
相關文獻
- 正常鼻咽部和鼻咽癌組織之末端酵素活性--初報
- 鼻咽癌末端酵素活性與其反轉錄酶mRNA表現之相關性
- The Role of CD 夰狇Cells and CD11a in the Rejection-Reaction of the Allogeneic Microskin Grafting
- Orbital Invasion in Nasopharyngeal Carcinoma: Evaluation with Computed Tomography and Magnetic Resonance Imaging
- 上頜掀開術切除局部復發之鼻咽癌--三例報告
- 中醫脈診儀應用於鼻咽癌患者接受放射線治療全程之研究
- Detection of Human Papilloma Virus and Epstein-Barr Virus DNA in Nasopharyngeal Carcinoma by Polymerase Chain Reaction
- 以螢光透視錄影吞嚥檢查評估鼻咽癌患者因放射線治療所致之吞嚥功能異常
- 鼻咽癌經放射線治療後的顳葉壞死
- 豬胸腺萃取物開發與活性測試
頁籤選單縮合
題名 | 正常鼻咽部和鼻咽癌組織之末端酵素活性--初報=Telomerase Activities in Tissues of Normal Nasopharynx and Nasopharyngeal Carcinoma--A Preliminary Report |
---|---|
作者 | 黃仲鋒; 卓忠隆; 郭士彰; 黃純真; 梁雲; 黃昭敏; 蘇志英; Hwang, Chung-feng; Cho, Chung-lung; Kou, Shih-chang; Huang, Shung-chen; Leung, Stephen-wan; Huang, Chao-ming; Su, Chih-ying; |
期刊 | 中華民國耳鼻喉科醫學雜誌 |
出版日期 | 19990300、19990400 |
卷期 | 34:2 民88.03-04 |
頁次 | 頁109-113 |
分類號 | 416.879 |
語文 | chi |
關鍵詞 | 鼻咽癌; 末端酵素; 淋巴細胞; Nasopharyngeal carcinoma; Telomerase; Lymphocyte; |
中文摘要 | 背景:末端染色體( telomere )是一種位於染色體末端的特殊構造,包含有數 百到三萬個重複的鹽基,它在人類正常分化的細胞持續分裂老化過程中會逐漸縮短;而末端 酵素( telomerase )其功能是合成末端的 DNA,本身是一種核酸蛋白質,在生殖細胞及大 部份惡性腫瘤組織中都存在。至於鼻咽癌與末端酵素的關係則少有報告。 方法:切片取得 18 件鼻咽癌及 32 件組織學正常之鼻咽組織並冷凍在 -70 ℃儲存, 檢體 經病理醫師檢查確定診斷,並以 TRAP 分析法測定末端酵素活性。 結果:18 件鼻咽癌除了 1 件以外,都表現出末端酵素活性。有趣的是 32 件無鼻咽癌病灶 之鼻咽組織中也有 26 件可偵測出末端酵素活性。6 件無末端酵素活性者,4 件是鼻咽癌經 放射治療後的組織,2 件是取自非病灶側之鼻咽癌病例。 結論:結果顯示末端酵素活性在鼻咽癌是很常見的。由於正常的鼻咽組織存在許多活化的淋 巴細胞,而活化的淋巴細胞會表現出末端酵素活性,因此正常的鼻咽組織也可偵測出末端酵 素。放射治療後的鼻咽組織由於缺乏活化淋巴系統所以無法發現此酵素活性。因此,我們建 議以此酵素研究鼻咽癌時,必須把上述之各種情況列入考量才可減少誤差。 |
英文摘要 | Background: Telomeres are specialized structures at the ends of chromosomes that consist of a few hundred base pairs to approximately 30 kbp. Reduction in average telomere length has been noted during normal aging in human differentiated cells. Activation of telomerase, a ribonucleoprotein that synthesizes telomeric DNA, is found in germ cells and most malignant tumors. However, there are few reports concerning about the relationship between telomerase activities and nasopharyngeal carcinoma (NPC). Methods: Tissue Samples from 18 nasopharyngeal carcinomas (NPC) and 28 histologically normal nasopharyngeal tissue samples, which were pathologically confirmed, were obtained as frozen specimens stored at -70 ℃. The telomerase activity was measured by telomeric repeat amplification protocol (TRAP) assay. Results: All 18 NPC tissues except one were telomerase-positive. Telomerase activity was detectable in 26 of 32 histologically normal nasopharyngeal tissues. Of the 6 telomerase-negative tissues, four were obtained from postirradiated, tumor-free nasopharynx and two from non-lesion sides of two NPC patients. Conclusions: Our results revealed that activation of telomerase activity was frequently present in NPC tissue. Detectable telomerase activities were observed in most normal nasopharyngeal tissues probably because there are many activated lymphocytes that retain telomerase activities. We could not detect telomerase activities in post-irradiated nasopharyngeal tissues whose lymphoid system had been destroyed by irradiation. Therefore, when we study the relationship between NPC and telomerase activities, it is important to take the above phenomenon into consideration. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。