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題名 | Effects of Copper on the Changes of Membrane Lipids, Photosynthetic Pigments and Thiol-containinng Compounds in Zinnia Elegans Leaves=銅對百日草葉中原生質膜的脂質、光合作用色素及硫化物含量的影響 |
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作者 | 馬筱筠; 陳榮銳; Ma, Shieu-yun; Chen, Yung-reui; |
期刊 | Taiwania |
出版日期 | 19990600 |
卷期 | 44:2 1999.06[民88.06] |
頁次 | 頁259-270 |
分類號 | 373.1 |
語文 | eng |
關鍵詞 | 銅; 膜脂; 色素; 含硫化合物; 毒害; 百日草; Copper; Membrane lipids; Pigments; Thiol-containing compounds; Toxicity; Zinnia elegans; |
中文摘要 | 五週大的百日菊幼苗以含或不含50μM硫酸銅的營養液培養後,以PEG-Dextran雙 相分配系純化原生質膜的方法,收集取樣分離與測試。經分析顯示Palmitic acid (16:0)、Linoleic acid (18:2)及Linolenic acid (18:3)為三種主要的脂肪酸,不飽和脂肪酸與飽含脂肪酸的比例因 銅處理而減低。銅處理也促進脂質過氧化物的生成和非蛋白質性的含硫化物的合成,同時造 成百日草幼葉中葉綠素及總類胡蘿蔔素的增加。 |
英文摘要 | Five-week-old plants of Zinnia elegans were grown in culture medium containing 50 μM CuSO . Plasma membrane was isolated and purified from both leaves of control and susceptible plants with method of polyethylene glycol/dextrin two-phase partition system. The major fatty acids of plasma membrane were identified as palmitic acid (16:0), linoleic acid (18:2) and linolenic acid (18:3). The ratios of unsaturated fatty acids to saturated fatty acids were reduced in copper-treated plants. The productions of lipid peroxides and nonprotein thiol-containing compound were obviously enhanced in copper-treated plants. Copper also caused the increment in chlorophyll content and total amounts of carotenoids in young leaves. |
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