查詢結果分析
來源資料
頁籤選單縮合
題名 | 裸鼠之育種繁殖及飼養= |
---|---|
作者 | 趙安基; |
期刊 | 醫學研究 |
出版日期 | 19910500 |
卷期 | 11:6 1991.05[民80.05] |
頁次 | 頁409-418 |
分類號 | 383.16 |
語文 | chi |
關鍵詞 | 育種; 飼養; 裸鼠; 繁殖; |
中文摘要 | 裸鼠(Nude mice)係一小鼠之突變種,因先天缺少胸腺,可利用其免疫缺陷進行人癌移植,及一些不易在普通動物進行的感染實驗。本裸鼠室自民國66年開始建立,經13餘年的育種、繁殖及飼養經驗、使原本生產後6月齡之裸鼠存活率由12%升至90%。初期在飲水中摻入高濃度之抗生素(ampicillin 500mg/l, tetracycline 750mg/l)及維他命,現因用福馬林燻煙及每週定期以噴火、殺菌液(0.1% benzalkonium chloride)消毒四周環境,保持無致病菌狀態,故目前飲水中停加抗生素,在飼養期間亦未發生感染及衰弱徵候。為了獲得最佳之產仔數、離乳率及產活率,我們比較四種不同交配方法產生之效果,第一種方法以雌雄裸屬交配,因雌裸鼠照顧幼鼠能力差並常加以吞食,其離乳率僅32-40%,故不宜採用雌裸鼠做為繁殖。第二種方法雌裸鼠配以有毛雄鼠(具裸鼠基因)得到之生產裸鼠率為47-52%而離乳率極低(31-33%)。第三種方法由具裸鼠基因之有毛雌雄鼠互相交配後,離乳率雖高達89-93%,然而獲得裸鼠之機率太低(17-23%)故也極少使用。第四種方法以具裸鼠基因之有毛雌鼠與雄裸鼠(nu/nu)交配,獲得裸鼠之機率(47-48%)雖較純雌雄裸鼠交配低,但離乳率高達93-94%,與其他方法比較呈明顯差別(P<0.001),故本室目前採用此方法。並將分娩後幼鼠瘦弱者,利用皮下注射人工營養劑(5%葡萄糖),加上餵食多種維他命(poly-vitamins),可使離乳率提高為98%。現最高繁殖量每年達5000隻,可供全國研究者使用。 |
英文摘要 | The nude mouse is a mutant species. Because nude mice inherently lack the thymus, we can take advantage of this immune defect to transplant human cancer or to conduct infection experiments that impossible to carry out on other animals. Our nude mice laboratory was first established in 1977, having accumulated 13 years of experience in their breeding, reproduction and raising. The survival rate of our nude mice 6 months after birth has risen from 12% to 90%. In the initial stage, we needed to add high doses of antibiotics (ampicillin 500 mg/l, tetracycline 750 mg/l) and vitamins to their drinking water. At present, owing to improvements in environment, we only use formalin to smoke the rooms and weekly use gun fire and 0.1% benzalkonium chloride solution to sterilize the environments. In addition, we regularly conduct bacterial colony tests of their environment and cultures of their excreta to assure a specific pathogen-free condition. In order to obtain the best results in newborn number, weaned rate and survival rate, we compared 4 different methods of mating. The first method was to use female and male nude mice. Because female nudes were poor nurses having tendency tend to eat their newborn and the weaned rate was only 32-34%, thus the results were not satisfactory. The intercourse between female nudes and male haired mice with nude gene (second method) resulted in low rate of newborn nudes (47-52%) and low weaned rate (31-33%). The third method involving mating male and female haired mice with nude gene produced high r=weaned rate (89-93%). However, the rate of obtaining newborn nudes was very low (17-23%). The best method was mating the haired females with nude gene to male nudes. Although this method produced lower rate of newborn nudes (47-48%) than the mating of pure male and female nudes (100%), the weaned rate was as high as 93-94%. Therefore, we recommend the use of the fourth method. If newborn nudes found to be weak and thin, we added artificial nutrition consisting of subcutaneous glucose (5%) and oral polyvitamins so as to attain a higher weaned rate up to 98%. The number of nude mice reproduced per year in our laboratory reached as high as 5,000, thereby enabling us to supply researchers throughout the country. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。