頁籤選單縮合
題名 | Preliminary Study on Cataractous Human Lenses Using Near-Infrared Fourier Transform Raman Spectroscopy= |
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作者 | 黃福永; 何愈; 許銘清; 翁鳳如; Huang, Fu-yung; Ho, Yuh; Hsu, Ming-ching; Wong, Fong-zo; |
期刊 | Journal of the Chinese Chemical Society |
出版日期 | 19990200 |
卷期 | 46:1 1999.02[民88.02] |
頁次 | 頁121-126 |
分類號 | 340 |
語文 | eng |
關鍵詞 | Near infrared fourier transform raman spectroscopy; Cataractous human lens; |
英文摘要 | Raman vibrations of the fingerprint of aromatic amino acid residues were analyzed to study the changes of cataractous lens protein in the cortex and nucleus at various ages. Tryptophan content, analyzed by the quantification of ratio, shows the damage (modification) of tryptophan residue in the nucleus is caused primarily by the formation of cataracts, not by the aging process. Micro-environmental changes of tryptophan and tyrosine were analyzed by the intensity ratios of I□/I□ and I □/I□ respectively. The decrease of the ratio of I□/I□, from 0.9 to 0.6 in the nucleus and from 0.7 to 0.6 for the cortex, reveal that more buried tryptophan residues become exposed in the cortex than in the nucleus during cataractogenesis, especially for non-senile cataractous lenses. The ratio of I□/I□ is around 1.0 for both cortical and nuclear proteins at various ages, indicating some tyrosine residues have undergone a change in their hydrogen bonding environment. When compared to previous studies, we found that a normal (clear) lens has a higher peak at 1617 cm□ than at 1604 cm□ while a dense opaque or brunescent lens shows stronger intensity at 1604 cm□ than at 1617 cm□, suggesting the ratio of I□/I□ can be used to evaluate the human lens morphology. |
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