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題名 | Expression of LDH-C Isozyme among Lizard Taxa: Evolutionary Implications for the Vertebrate Lactate Dehydrogenase Gene Family=C型乳酸去氫酶(LDH-C)在蜥蜴之表現:對脊椎動物乳酸去氫酶基因族在演化上之涵義 |
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作者 | 郭建賢; 高善 ; 翁慶豐; 李信徹; Kuo, Chien-hsien; Kao, San; Weng, Ching-feng; Lee, Sin-che; |
期刊 | 動物研究學刊 |
出版日期 | 19990700 |
卷期 | 38:3 1999.07[民88.07] |
頁次 | 頁344-349 |
分類號 | 388.7921 |
語文 | eng |
關鍵詞 | 蜥蜴; 乳酸去氫酶; 演化; 有羊膜類; Lizard; Lactate dehydrogenase; Evolution; Amniote; |
中文摘要 | 為了更完整的瞭解蜥蜴類之乳酸去氫�t同功�t的表現,以澱粉凝膠電泳法進行實驗,共分析 Hemidactylus frenatus, Japalura mitsukurii, Takydromus formosanus, Eumeces elegans, Mabuya longicaudata, Sphenomorphus indicus, 及Scincella formosensis等四科七屬之蜥蜴。分別就蜥蜴的腦、眼、心臟、肝臟、骨骼肌和生殖腺分析乳酸去氫�t同功�t表現的型式。發現蜥蜴除了具有與其他脊椎動物相同的A、B兩個基本的酵素外,在H. frenatus, E. elegans及M. longicaudata三種蜥蜴的睪丸中發現第三種型式的乳酸去氫�t(LDH-C)的表現。此睪丸專一的酵素產物在陽極的移動上較其他乳酸去氫�t同功�t快。由此一發現我們推斷睪丸專有的乳酸去氫�t(LDH-C)是由有羊膜類祖先之乳酸去氫�tA型(LDH-A)所衍生出來的。 |
英文摘要 | In order to more completely understand the complete basis for the multiple LDH isozymes in lizards, seven species of Taiwanese lizards belonging to 4 families and 7 genera were sampled. Starch gel electrophoretic patterns of lizard LDH isozymes from brain, eye, heart, liver, muscle, and testis were analyzed. Like all other vertebrates, lizards possess the 2 fundamental LDH loci--A and B. A 3rd locus, LDH-C, was detected only in testes of Hemidactylus frenatus, Eumeces elegans, and Mabuya longicaudata. This testisspecific product, designated according to tissue-specific expression exhibited, a faster anodal migration than did the other LDH isozymes. These findings suggest that the testis-specific LDH isozyme was derived from ancestor amniote LDH-A. |
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