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頁籤選單縮合
題名 | 中藥製劑中摻加Fluoxymesterone, Methyltestosterone及Testosterone之高效液相層析法定量探討=Determination by High Performance Liquid Chromatography of Fluoxymesterone, Methyltestosterone and Testosterone in Adulterated Chinese Herbal Preparations |
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作者 | 顧瑞; 蔡明哲; 溫國慶; Ku, Yoe-ray; Tsai, Ming-jer; Wen, Kuo-ching; |
期刊 | 藥物食品分析 |
出版日期 | 19970600 |
卷期 | 5:2 1997.06[民86.06] |
頁次 | 頁121-129 |
分類號 | 414.92 |
語文 | chi |
關鍵詞 | 中藥摻加西藥檢驗; 高效液相層析; 男性荷爾蒙; HPLC; Chinese herbal preparation; Fluoxymesterone; Methyltestosterone; Testosterone; Adulterant; Androgen; |
中文摘要 | 本研究利用高效液相層析方法,分析添加於中藥劑之三種fluoxymesterone、 methyltestosterone 及 testosterone 男性荷爾蒙成分之可行性。本方法採用 C �痚f相層 析管柱,以水:乙�� (3:2) 沖提。結果顯示,三種西藥成分之檢量線於 10 ~ 160 μ g/ml 濃度之線性相關係數介於 0.9997 至 0.9999 間,顯示良好之線性關係。模擬添加於八珍湯 之回收率依序為 fluoxymesterone 87.7%;methyltestosterone 99.3%; 及 testosterone 91.1%,顯示準確性尚可接受。 最低檢出濃度介於 0.05 μ g/ml 至 0.10 μ g/ml 間。同 日內及異日間之相對標準差分別為 0.31 ~ 2.01%,0.95 ~ 5.11%, 顯示再現性亦可接受。 依此方法分析本局受理檢驗之四種自稱為鄨鞭、蛇鞭膠囊中檢出 methyltestosterone 之情 況, 其添加回收率分別為 95.4、 83.3、 90.7 及 88.8%。 至於該四種膠囊所含 methyltestosterone 之量介於 1.5 ~ 4.0% 之間。 換算成單位含量則 12 粒膠囊檢體中每 粒 methyltesterone 之含量介於 7.5 mg 至 19.7 mg 之間。 |
英文摘要 | A Chinese herbal preparation was assayed simultaneously by high performance liquid chromatography on an ODS column for the presence of adulterated three androgens, fluoxymesterone, methyltestosterone and testosterone. This chromatographic method was carried out using isocratic elution with water and acetonitrile (3:2). Calibration graphs of the three adulterants were constructed in the range 10-160 μ g/ml and their correlation coefficients were in the range 0.9997-0.9999. When these three androgens were added to Ba-Jen-Tang, the recoveries were 87.7% for fluoxymesterone, 99.3% for methyltestosterone and 91.1% for testosterone. The relative standard deviations for intraday and interday analyses were 0.31-2.01% and 0.95-5.11% (n=6), respectively. Four kinds of capsules labeled snake penis (Samples A-D) were consigned during Aug. 1995 and Jul. 1996. The methyltestosterone in the samples was assayed by the HPLC method described above. The recoveries of methyltestosterone in samples A, B, C and D were 95.4, 83.3, 92.7 and 88.8%, respectively. The content of methyltestosterone in these capsules ranged from 1.5 to 4.0% (7.5-19.7 mg in each capsule). Caffeine, diprophylline and three water soluble vitamins (ascorbic acid, nicotinamide and thiamine) were also detected in Samples A and B, and thiamine was detected in Samples C and D. The identity of these chemicals was confirmed by TLC and UV. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。