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題名 | 豬皮膠原蛋白膜之生物毒性與酶分解速率之研究=In Vitro Tests of Porcine Dermal Collagen Membranes--Biocompatibility and Enzymatic Degradation Rate |
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作者 | 呂炫堃; 林峰丕; 吳銘芳; | 書刊名 | 中華牙醫學雜誌 |
卷期 | 17:1 1998.03[民87.03] |
頁次 | 頁15-22 |
分類號 | 416.9 |
關鍵詞 | 豬皮膠原蛋白膜; 非活體細胞培養; 膠原蛋白酵素; Porcine dermal collagen membrane; In-vitro cell culture; Collagenase; |
語文 | 中文(Chinese) |
中文摘要 | 經由先遣之實驗,我們已證實經特殊製備之豬皮膠原蛋白膜( PDCM ),於活體 中具備相當之生物相容性與生物可吸收性。本研究之目的即希望進一步以非活體實驗檢測: ( 1 )於細胞培養之層次下,經戊二醛( GA )交鏈化之 PDCM,是否仍具有生物相容性; ( 2 )不同程度聚合之 PDCM,戊二醛是否會影響其酵素分解速率。於第一部分實驗,將已 製備完成之 PDCM ( 10 × 10 × 0.1mm )以 GA 三種濃度( 0.01%, 0.05%,3.00% ) 及未聚合之 PDCM (控制組)分成四組,與人類牙周韌帶纖維母細胞( HPLF )和人類牙齦 纖維母細胞( HGF )共同培養一、三、七天以後, 以敘述性方式記錄並評估 PDCM 之生物 相容性。 本宙驗之第二部分, 即以細菌膠原蛋白酵素( collagenase type Ⅰ, Clostridium histolyticum )溶解上述四組樣本,並計算其分解速率,製成比較圖。 結果 顯示,無論是控制組或其他三組,PDCM 對 HPLF 與 HGF 之細胞培養皆不具細胞毒性。以細 菌膠原蛋白酵素分解 PDCM 則發現:隨戊二醛修飾濃度之提高, PDCM 被酵素完全分解所須 之時間亦隨之增加。 僅 3.00% 戊二醛組之 PDCM 與其他三組比較,具有明顯之差異性。綜 上所述,本研究證明經特殊製備之 PDCM 對人類之牙周與牙齦纖維母細胞不具細胞毒性;吾 人可經由戊二醛之濃度來控制 PDCM 之酵素分解抗性; PDCM 將可使用於各種臨床用途上, 如牙周導引再生手術。 |
英文摘要 | From previous in-vivo study, we found that improved procine dermal collagen membrane (PDCM) developed in our laboratory is relatively biocompatible and biodegradable. In the first part of the present study, cell cultures of human periodontal ligament fibroblasts (HPLF) and human gingival fibroblasts (HGF) were used to investigate the cytotoxic effect of glutaldehyde (GA)-conditoned PDCM. The phynotypic appearance of HPLF and HGF in cell cultures were studied to evaluate the cytotoxicity of PDCM. Secondly, bacterial collagenase (collagenase type Ⅰ from Clorstrudium histolyticum) was applied to digest PDCM and clarify the effect of GA on the degradation rate of PDCM. After processing PDCM (10×10×0.1 mm) by a specific laboratory protocol it was air dried and cross-linked with 0.01%, 0.05% or 3.00% GA. Non-cross linked PDCM was served as the control. Our results indicated that when HPLFs or HGFs were cocultured with PDCM conditioned with 0.01%, 0.05% and 3.00% GA, the fibroblasts grew well and no retardation of growth or cell death was found. This suggests that the GA-conditioned PDCM has excellent biocompatibility and no cytotoxic effect on HPLFs or HGFs. As the concentration of GA increased, the enzymatic degradation rate of PDCM decreased conversely, but only that of the 3% GA group differed significantly from those of the other 3 groups. Because PDCM is non-cytotoxic and has a controllable resorption rate, we feel that it can be used in a guided tissue regeneration technique. |
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