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題名 | 應用雙套式聚合酶鏈反應快速鑑定傷寒沙門桿菌=Rapid Identification of Salmonella typhi by Duplex Polymerase Chain Reaction |
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作者 | 林建谷; Lin, Chien-ku; |
期刊 | 弘光學報 |
出版日期 | 19981000 |
卷期 | 32 1998.10[民87.10] |
頁次 | 頁81-96 |
分類號 | 415.2746 |
語文 | chi |
關鍵詞 | 雙套式PCR; 傷寒沙門桿菌; Hl-d鞭毛抗原; Vi抗原; Duplex PCR; Salmonella typhi; Hl-d flagellin; Vi antigen; |
中文摘要 | 傳統以生化型及血清型試驗鑑定傷寒沙門桿菌之過程相當煩雜且費時,因此發展 傷寒沙門桿菌之快速鑑定方法,在醫療及疫情防治工作上均有其必要性。本研究針對傷寒沙 門桿菌是沙門桿菌屬中唯一同時具有 H1 - d 鞭毛基因及 Vi 毒力基因之血清型特性,發展 特異性同時擴增此二基因之雙套式聚合 鏈反應( PCR )。 所測試之傷寒沙門桿菌以 H1 - d 基因特異性引子組( HD3 / HD4 )及 Vi 基因特異性引子組( P11F / P11R )進行雙 套式 PCR, 均可產生產物大小分別為 280bp 及 206bp 之產物:而其他僅具 H1 - d 基因 ( S. muenchen, S. florida 等)或 Vi 基因( S. paratyphi C, S. dublin 等)之菌株 ,則只產生個別 PCR 產物,此結果顯示此雙套式 PCR 系統在快速鑑定傷寒沙門桿菌之特異 性甚佳。 |
英文摘要 | The conventional biochemical and serological method for identification of Salmonella typhi is tedious and time-consuming. It is essential for medical remedy and epidemic control of typhoid infection by rapid and specific detection of S. typhi. Among Salmonella serotypes, S. typhi is the only one with both of H1-d and Vi antigens. Therefore, this study was to develop a duplex PCR system for specific identification of S. typhi through simultaneous amplification of genes encoding H1-d and Vi antigens. The PCR products based on amplification of HI-d and Vi genes by HD3/ HD4 plus P11F/P11R primer set were 280 bp and 206 bp, respectively. While, other strains with H1-d or Vi gene could only generated corresponding PCR product. These results showed such duplex PCR system was specific for the identification of S. typhi. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。