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頁籤選單縮合
題名 | 溶液中之常溫磷光=Room Temperature Phosphorescence in Solutions |
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作者 | 林萬寅; | 書刊名 | 化學 |
卷期 | 55:4 1997.12[民86.12] |
頁次 | 頁59-64 |
專輯 | 溶液態光物理化學 |
分類號 | 341.393 |
關鍵詞 | 微胞; 環糊精; 蛋白質; 常溫磷光; Micelle; Cyclodextrin; Protein; Room temperature phosphorescence; |
語文 | 中文(Chinese) |
中文摘要 | 微胞和環糊精之常溫磷光通常可以在去氧的溶液中及添加重原子物質的條件下被 偵測到。這兩種介質提供了一個較為隔離及剛性的環境以隔離外在淬熄劑、降低非輻射緩解 速率、提升磷光放射速率並藉著重原子之添加來提升系統間穿越之速率以利於常溫磷光之偵 測。此外,添加強電解質可以形成聚集數較大、堆積較緊密、剛性及微黏性增加、極性降低 之棒形微胞更有利於常溫磷光之偵測。由於微胞和環糊精常溫磷光之偵測簡單、選擇性好、 靈敏度高,非常適合用於檢測微量之芳香族化合物及作為 HPLC 之偵測。在去氧的水溶液中 ,大部份的蛋白質都可以顯現常溫磷光。由於蛋白質之 Trp 常溫磷光具有很窄之 0-0 峰而 且其磷光壽命與 Trp 所處之環境有很大的關運, 所以可以用於偵測蛋白質之結構、動性及 構形變化之動力學。 |
英文摘要 | Room temperature phosphorescence (RTP) can easily be observed in deoxygenated micelles and cyclodextrin solutions in the presence of heavy atoms. These two organized media provide an isolated and rigid environment to protect the luminophore from externalquenchers, to reduce the nonradiative decay rate and to increase the phosphorescence quantum yield. The addition of heavy atoms enhance the rate of intersystem crossing and hence the population of the triplet state to facilitate the detection of RTP. In the presence of a strong electrolyte, the micellar assembly may undergo a sphere-to-rod transition, which is usually accompanied by an increase in aggregation number, rigidity, microviscosity and a decrease ini polarity. These factors favor the observation of RTP in rodlike micelles. Due to its simplicity, good selectivity and sensitivity, RTP in micelles and cyclodextrin is useful for the trace analysis of aromatic compounds and of HPLC detection. In deoxygenated aqueous solution, most proteins exhibit RTP for the amino acid buried in an isolated and rigid core. Trp phosphorescence in proteins shows a well-resolved 0-0 band and an environment-dependent lifetime. RTP in proteins is a very sensitive probe for the conformational states, the structural changes and dynamics of proteins. |
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