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題名 | 暫態回應法在酵素光學分割動力學研究上之應用=Application of Transient Response Method to the Kinetic Study of Enzymatic Resolution |
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作者 | 吳昭燕; | 書刊名 | 義守大學學報 |
卷期 | 4 1997.08[民86.08] |
頁次 | 頁311-318 |
分類號 | 346.2 |
關鍵詞 | 暫態回應法; 酵素光學分割; 動力學; 力矩法; Transient response method; Enzymatic resolution; Kinetics; Moment analysis; |
語文 | 中文(Chinese) |
中文摘要 | 酵素光學分割法被視為目前製造純光學化合物最具潛力之方法,唯其動力學研究 之進行相當繁複。本文首次嘗試將暫態回應法引入酵素光學分割系統之動力學研究中,文 中除了對反應器之型態及實驗進行之方式提出建議外,並建立其理論模式及相對應之力矩 ( moments) 以進行數據分析。 比之於傳統方法,本方法因設備及操作較為簡單,且因所需時 間較少而無酵素活性衰退之問題,可謂為進行酵素光學分割動力學研究之利器。 |
英文摘要 | A transient response method is suggested here for the kinetic study of an enzymatic resolution system. The theoretical model dealing with the kinetic behavior of the reactor responding to the proposed experimental methods was established. The moments, which provide the information about the individual rate constants of each enantiomer. were then derived. From the moments of the response curves of the enantiomeric substrates, it was shown that the reaction constants of the enzyme can be easily obtained provided the enzyme is in excess. Because the apparatus and operation are simple, the time required is short, and the inactivation of the enzyme is not a problem any more, the technique presented here could be very attractive for the kinetic studies of enzymatic kinetic resolution system. |
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