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題名 | Population Cell Differentiation of Serratia Marcescens on Agar Surface and in Broth Culture=Serrtia Marcescens在固體及液體培養基中之族群細胞分化行為 |
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作者 | 賴信志; 賴孟君; 林淑端; 陸坤泰; 何憲武; | 書刊名 | 中華民國微生物及免疫學雜誌 |
卷期 | 30:4 1997.11[民86.11] |
頁次 | 頁242-254 |
分類號 | 364.6 |
關鍵詞 | 液體培養基中; 群細胞分化行為; Bacterial differentiation; Swarming; LuxAB reporter genes; Scrratia marccsccns; |
語文 | 英文(English) |
中文摘要 | Serratia marcescens在LB培養基上顯示族群表面移行(swarming)現象,並且於移行族群之前端可發現分化之細胞。Sarming 之機制目前前並不清楚,也不知道分化之細胞是否可於液體培養基中觀查到。為探討此問題,lux AB 冷光基因被接於hag基因啟動子之後作為報導基因 (reporter gene)。 藉由X光底片及冷光計來偵測光之強弱,可以監控細胞之分化情形。結果顯示細胞族群在固體培養基上依不同的時間及空間而分化,另外在固體及液體培養基中生長之細胞的分化有相似情形,顯示固體表面接觸並不是引起細胞分化之必要因子。 |
英文摘要 | The bacterium Serratia marcescens shows population surface migration (swarming) phenomenum on an LB swarming plate, and differentiated cells can be observed at the swarming front. How the cell population differentiates during swarming on the agar surface is not known, neither is it clear whether cells with differentiated characteristics can be observed in broth culture. To monitor the population cell differentiation in a highly sensitive way without cell destruction, experiments were designed using bacterial luciferase genes luxAB as the reporter genes to allow direct monitoring of the differentiating cells through bioluminescence. An isogenic S. marecscens strain was constructed with luxAB under the control of the promoter of flagellin gene hag phag::luxAB). Patterns of cell differentiation were monitored either by direct X-ray film exposure and/or by Autolumat luminometer detection. Results show that population cell differentiation on the agar surface occurs first in a temporal and then spatial way during colonial growth. It was also found that cells harvested from both the spreading agar plate and broth culture showed differentiation patterns similar to those from swarming cells, suggesting that the agar surface culture may not be essential for the formation of differentiated cells. |
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