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題 名 | 犬圍肛腺腫瘤之AgNORs之變化研究=Nucleolar Organizer Regions(NORs)in Canine Perianal Gland Tumors |
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作 者 | 洪鈴柱; 朱瑞民; 龐飛; 鄭謙仁; 王汎熒; | 書刊名 | 中華民國獸醫學會雜誌 |
卷 期 | 23:5 1997.10[民86.10] |
頁 次 | 頁403-410 |
分類號 | 437.66 |
關鍵詞 | 犬; 圍肛腺腫瘤; Dog; Perianal gland tumors; AgNORs; |
語 文 | 中文(Chinese) |
中文摘要 | 本方法乃應用鍍銀染色法染出核仁機化體區之相關蛋白質(argyrophilic staining of nucleolar organizer regions-associated proteins, 簡稱 Ag-NORs) 以評 估腫瘤的回變程度或是惡性程度。 其原理為利用核仁內的非組織相關蛋白質 (non-histon associated proteins) 之嗜銀性性質而予以染色的; 這些蛋白質位於核糖體 DNA(ribosomal DNA) 轉錄活化的位置, 因而稱為核仁機化體區 (nucleolar organizer regions,簡稱 NORs), 也被認為是蛋白質合成及細胞增殖的標示,故近年來被嘗試用於腫 瘤之良惡性的判斷。 本研究在利用石臘包埋的組織之 AgNOR 的計數,評估其應用於犬圍肛 腺腫瘤診斷的價值。 實驗中使用犬圍肛腺有關的組織標本共 16 個,包括 3 個正常犬圍肛 腺組織,4 個圍肛腺增生組織,6 個圍肛腺瘤及 3 個圍肛腺癌。 研究的方法包括:組織學 分類、 計算每個細胞含有 AgNORs 的平均值、AgNUs (Ag NORs 在細胞核內聚集成緊密的小 群數 ) 的平均值、有絲分裂的數目、儲備細胞 (reserve cells) 所佔的千分比值、年輕細 胞 (juvenile cells) 所佔的千分比值、及肝樣細胞所佔的千分比值等。 結果發現 AgNORs 平均值在區別增生組織、 腺瘤、 及腺癌上有顯著性差異。 犬圍肛腺癌的AgNORs平均值 (9.31 ± 0.41) 極顯著地高於腺瘤的 AgNORs 平均值 (6.29 ± 0.66;P=0.0002);腺瘤的 AgNORs 的平均值 (6.29 ± 0.66) 也顯著高於增生組織 (5.22 ± 0.43;p=0.0211)。在 線性迴歸分析上:AgNORs 的平均值與 AgNUs 的平均值、儲備細胞的千分比值、年輕細胞比 值、肝樣細胞的千分比值、及有絲分裂值等均有顯著性相關 (P 值均小於 0.01)。由以上結 果,可了解組織細胞之 Ag NOR 計數分析是一可資用以區別犬圍肛腺增生及良、惡性腫瘤之 簡易方法。它可使用於福馬林固定之石蠟包埋的組織,並且費用亦不昂貴。因此可為作病理 診斷工作者一個對惡性程度較為客觀的評級方法。 |
英文摘要 | The purpose of this investigation is to evaluate the application of Ag- NOR technique to the level of anaplasia of a tumor. The basis of this method is the argyrophilic staining of intranucleolar, non-histon proteins in paraffin-embedded tissues which are specifically associated with transcriptionally active sites of ribosomal DNA and named as nucleolar organizer regions. They can be considered as some markers for the protein synthesis and thus the proliferation rate of a given cell. In this study 3 normal canine perianal glands, 4 hyperplasias, 6 adenomas and 3 adenocarcinomas were used. The following parameters were used: the histological grade, the mean AgNORs count, the mean AgNUs (AgNORs were aggregated as tight clusters within the nucleolus) count, the mitotic count, the percentage of juvenile cell (cells of intermediate maturation),the percentage of hepatoid cell, and the percentage of reserve cell in a total of 1,000 cells presented in the same tumor areas. However, only the mean AgNORs count, distinguished more clearly between the malignant (adenocarcinomas) and the benign (hyperplasias and adenomas) proliferative lesions. The mean AgNORs count (mean 9.31 ± 0.41) in adenocarcinoma was significantly higher than in adenoma (mean 6.29 ± 0.66 P=0.0002), and the mean AgNORs count (mean 6.29 ± 0.66) in adenoma was significantly higher than in hyperplasia (mean 5.22 ± 0.43, P=0.0211). The mean number of AgNORs correlated closely with the mean number of AgNUs, the percentage of juvenile cell, the percentage of hepatoid cell and the number of mitoses. In conclusion, the Ag-NOR technique is a simple, inexpensive and accurate method which can be applied to formalin fixed, paraffin-embedded tissues as a marker of malignancy for a diagnostic pathologist. |
本系統中英文摘要資訊取自各篇刊載內容。