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| 題 名 | 補骨脂水萃取物及補骨脂酚對大白鼠初代肝細胞之抗氧化保護作用=Antioxidative Protection of Water Extract of Psoralea Fruit and Bakuchiol on Primary Rat Hepatocytes |
|---|---|
| 作 者 | 葉佳聖; 沈立言; 李宗貴; 蔡順仁; | 書刊名 | 中華民國營養學會雜誌 |
| 卷 期 | 22:3 1997.08[民86.08] |
| 頁 次 | 頁303-324 |
| 分類號 | 415.121 |
| 關鍵詞 | 補骨脂; 補骨脂酚; 氧化緊迫; 麩胱甘冴; Psoralea fruit; Bakuchiol; Oxidative stress; Glutathione; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 以由大白鼠分離之初代肝細胞為實驗模式探討在葡萄糖╱葡萄糖氧化 (glucose╱glucose oxidase)誘發之氧化緊迫(oxidative stress)下補骨脂水萃取物及補骨脂酚對肝細胞生存力之保護作用,並分析其對肝細胞脂質過氧化(TBARS)、麩胱甘 (glutathione, GSH)濃度與麩胱甘 相關代謝酵素活性之影響。肝細胞在有或無補骨脂水萃取物(0.6 mg╱mL)及補骨脂酚(0.125 mM)添加條件下培養,除空白組外,均加入50μg╱mL葡萄糖及100 μg╱mL葡萄糖氧化 。結果顯示補骨脂水萃取物或補骨脂酚顯著抑制乳酸去氫 滲漏率(lactate dehydrogenase leakage);經過8小時處理後,乳酸去氫 滲漏率為對照組之1╱2左右,而此保護作用並持續至48小時(p<0.05)。至於肝細胞脂質過氧化現象,補骨脂水萃取物或補骨脂酚可顯著(p<0.05)抑制葡萄糖╱葡萄糖氧化 誘導之脂質過氧化。另一方面,補骨脂水萃取物或補骨脂酚有助於維持氧化緊迫下肝細胞內還原態及氧化態麩胱甘 之濃度,甚至誘導更高的還原態麩胱甘 之濃度。在肝細胞內麩胱甘 之相關代謝酵素活性方面,補骨脂水萃取物或補骨脂酚可減少麩胱甘 相關代謝酵素如麩胱甘 過氧化 、硫轉移及還原 活性因氧化緊迫造成之不降,甚至提升其活性。由此可知,補骨脂水萃取物及補骨脂酚藉由提升肝細胞之麩胱甘 濃度及其相關代謝酵素活性,保護肝細胞免於氧化傷害。 |
| 英文摘要 | Protectiev effect of water extract of psoralea fruit and bakuchiol on the viability, lipid peroxidation, glutathione (GSH) leveles and activities of GSH-related enzymes of primary rat hepatocytes under oxidative stress induced by glucose/glucose oxidase was investigated. Hepatocytes were incubated with or without water extract of psoralea fruit (0.6 mg/mL) or bakuchiol (0.125 mM), and 50μg/mL glucose, 100μg/mL glucose oxidase except for the blank. The results reveal that the lactate dehydrogenase leakage of hepatocytes incubated with water extract of psoralea fruit or bakuchiol was significantly suppressed; showing only about half that of the control at 8 hr incubation, and the protective effects lasted till 48 hr (p<0.05). As for the lipid peroxidation of hepatocytes, water extract of psoralea fruit or bakuchiol significantly (p<0.05) controlled the lipid peroxidation induced by glucose and glucose oxidase. On the other hadn, water extract of psoralea fruit or bakuchiol helped maintain GSH and oxidized glutathione levels in hepatocytes under oxidative stress, and even induced more GSH production. Regarding the aspect of the GSH-related enzymes activities, water extract of psoralea fruit or bakuchiol stopped the activities of glutathione peroxidase, gluathinoe S-transferase and glutathione reductase decrease what casued by oxidative stress and even to elevate the activities of them. Thus, water extract of psoralea fruit and bakuchiol protected hepatocytes from oxidation damage via increasing the GSH levels and elevating the activities of GSH-related enzymes of hepatocytes. |
本系統中英文摘要資訊取自各篇刊載內容。